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- AAT Bioquest
- AAT-22605
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- 2025年10月19日
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- 文献和实验
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- 库存：
大量
- 英文名：
Live or Dead™ Fixable Dead Cell Staining Kit
- CAS号：
/
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
200 Tests
关键词：Live或Dead 可固定死细胞染色试剂盒 NIR荧光详细如下
Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to uniformly label fixed mammalian cells in infrared fluorescence for flow cytometry applications with red laser excitation. The kit uses a proprietary red fluorescent dye that is more fluorescent upon binding to cellular components. The fluorescent dye used in the kit is well excited with the red (635 nm) to fluorescence at 775 nm (APC/CY7 channel). The kit provides all the essential components with an optimized cell-labeling protocol. It is an excellent tool for preserving of fluorescent images of particular cells, and can also be used for fluorescence flow cytometry applications.
Components
Component A: Stain It™ NIR fluorecence 1 vial
Component B: DMSO 1 vial (200 µL)
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
| 货号 | 英文名称 | 规格 |
| AAT-104550A0 | iFluor™ 560 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550A1 | iFluor™ 560 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550B0 | iFluor™ 568 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550B1 | iFluor™ 568 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550C0 | iFluor™ 594 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550C1 | iFluor™ 594 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550D0 | iFluor™ 610 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550D1 | iFluor™ 610 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550E0 | iFluor™ 633 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550E1 | iFluor™ 633 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550F0 | iFluor™ 647 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550F1 | iFluor™ 647 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550G0 | iFluor™ 660 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550G1 | iFluor™ 660 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550H0 | iFluor™ 670 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550H1 | iFluor™ 670 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550I0 | iFluor™ 680 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550I1 | iFluor™ 680 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550J0 | iFluor™ 700 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550J1 | iFluor™ 700 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550K0 | iFluor™ 710 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550K1 | iFluor™ 710 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550L0 | iFluor™ 750 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550L1 | iFluor™ 750 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550M0 | iFluor™ 790 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550M1 | iFluor™ 790 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550N0 | iFluor™ 800 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550N1 | iFluor™ 800 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550O0 | iFluor™ 810 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550O1 | iFluor™ 810 Anti-human CD45 Antibody *HI100* | 500tests |
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文献和实验实验室中时常听到这样的困惑，「明明通过免疫荧光（IF）能看到目的蛋白表达在细胞核膜上的表达，但为什么不论用总蛋白还是核蛋白进行 WB 检测，都无法检测到目的蛋白？」 类似这样的问题，你是否也深有感触？ 实际操作过程中，是否遇到过免疫荧光有结果而蛋白印迹却没条带的情况？什么原因导致了这种情况？又该如何处理？ 一、IF & WB 免疫荧光（IF）和免疫印迹（WB）都是利用抗原和抗体的特异性结合对目的蛋白质进行检测。IF 采用抗体和荧光检测固定细胞或组织中目的
通用，尤其是在进行动态检查时。医琳师妹：我将我所作的方法与大家共享：吖啶橙/溴化乙锭双荧光染色测定PC12细胞凋亡（acridine orange/ethidium bromide, AO/EB）：（1）细胞爬片：在6孔培养板中预先置入玻璃盖玻片，接种细胞悬液，干预后，予95%乙醇固定15分钟，微干，然后准备荧光染色。将100mg/L溶于PBS的吖啶橙和100mg/L溶于PBS的溴化乙锭各5μl（临用前混合加入，加样量宜小，有时各2μl-5μl足够，量太大容易形成细胞凋亡的假阳性），在照相前混匀
Q：Annexin V 凋亡检测试剂盒的原理是什么？ A：Annexin V 是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸 PS 有高度亲和力，可通过细胞外侧暴露的 PS 与凋亡早期细胞胞膜结合，将 Annexin V 标记荧光染料如 FITC 利用流式细胞仪或荧光显微镜可检测细胞凋亡。 碘化丙啶(Propidium Iodide, PI)或 7-AAD 可与双链 DNA 特异性结合，并产生强烈的荧光，正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性，核染料 PI 或 7-AAD
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