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- 库存：
大量
- 英文名：
DAX-J2™ Orange
- CAS号：
/
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
1 mg
齐源生物专业代理美国AAT Bioquest产品，并有大量储存现货欢迎致电
关键词：一氧hua氮NO荧光探针 DAX-J2 橙详细如下
DAX-J2™ Orange is a new nitric oxide (NO) sensor recently developed by AAT Bioquest. It is a non-fluorescent cell permeable reagent that can measure free NO and nitric oxide synthase (NOS) activity in living cells under physiological conditions. Once inside the cell the blocking groups on the DAX-J2 reagent are released to generate the highly fluorescent product upon NO oxidation. DAX-J2 fluorescent product can be detected using the filter set of Cy3® and TRITC that is equipped with most of flow cytometers and fluorescence microscope. DAX-J2 Orange has distinct advantages for NO detection than the popular DAF-2 NO probe: 1). It does not require esterase activity for NO detection. DAF-2 requires intracellular esterases to cleave its acetate groups for detecting NO activity. This esterase dependence often complicates the NO detection since esterse activities are affected by cell health and many other factors. 2). DAX-J2 product exhibits pH-independent fluorescence while DAF-2 has its fluorescence highly affected by pH. 3). DAX-J2 fluorescent product is much more photostable than DAF-2 product.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
关键词：一氧hua氮NO荧光探针 DAX-J2 橙详细如下
DAX-J2™ Orange is a new nitric oxide (NO) sensor recently developed by AAT Bioquest. It is a non-fluorescent cell permeable reagent that can measure free NO and nitric oxide synthase (NOS) activity in living cells under physiological conditions. Once inside the cell the blocking groups on the DAX-J2 reagent are released to generate the highly fluorescent product upon NO oxidation. DAX-J2 fluorescent product can be detected using the filter set of Cy3® and TRITC that is equipped with most of flow cytometers and fluorescence microscope. DAX-J2 Orange has distinct advantages for NO detection than the popular DAF-2 NO probe: 1). It does not require esterase activity for NO detection. DAF-2 requires intracellular esterases to cleave its acetate groups for detecting NO activity. This esterase dependence often complicates the NO detection since esterse activities are affected by cell health and many other factors. 2). DAX-J2 product exhibits pH-independent fluorescence while DAF-2 has its fluorescence highly affected by pH. 3). DAX-J2 fluorescent product is much more photostable than DAF-2 product.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
| 货号 | 英文名称 | 规格 |
| AAT-22803 | Cell Meter™ NIR Mitochondrion Membrane Potential Assay Kit *Optimized for Microplate Reader* | 500Tests |
| AAT-22804 | Cell Meter™ Mitochondrion Membrane Potential Assay Kit *Orange Fluorescence Optimized for Flow Cytometry* | 100Tests |
| AAT-22805 | Cell Meter™ Mitochondrion Membrane Potential Assay Kit *Orange Fluorescence Optimized for Microplate Reader* | 500Tests |
| AAT-22810 | Cell Meter™ Intracellular GSH Assay Kit *Optimized for Flow Cytometry* | 100Tests |
| AAT-22811 | Cell Meter™ Nuclear Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry* | 100Tests |
| AAT-22812 | Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Blue Fluorescence* | 200tests |
| AAT-22813 | Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Blue Fluorescence* | 200Tests |
| AAT-22816 | Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Red Fluorescence* | 100Tests |
| AAT-22817 | Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Red Fluorescence* | 100Tests |
| AAT-22820 | Cell Meter™ Multiplexing Caspase 3/7, 8 and 9 Activity Assay Kit *Triple Fluorescence Colors* | 100Tests |
| AAT-22821 | Cell Meter™ Generic Fluorimetric Caspase Activity Assay Kit *Green Fluorescence Optimized for Flow Cytometry* | 100Tests |
| AAT-22822 | Cell Meter™ Generic Fluorimetric Caspase Binding Assay Kit *Red Fluorescence Optimized for Flow Cytometry* | 100Tests |
| AAT-22823 | Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry* | 100Tests |
| AAT-22824 | Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry* | 100Tests |
| AAT-22825 | Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Orange Fluorescence Optimized for Flow Cytometry* | 100Tests |
| AAT-22826 | Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Red Fluorescence Optimized for Flow Cytometry* | 100Tests |
| AAT-22827 | Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry* | 100Tests |
| AAT-22828 | Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm* | 100Tests |
| AAT-22829 | Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Excited at 405 nm* | 100Tests |
| AAT-22830 | Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Orange Fluorescence Excited at 405 nm* | 100Tests |
| AAT-22831 | Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry* | 100Tests |
| AAT-22832 | Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry* | 100Tests |
| AAT-22835 | Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm* | 100Tests |
| AAT-22836 | Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Green Fluorescence Excited at 405 nm* | 100Tests |
| AAT-22837 | Cell Meter™ APC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry* | 100Tests |
| AAT-22838 | Cell Meter™ PE-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry* | 100Tests |
| AAT-22840 | Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit I *Triple Fluorescence Colors* | 100Tests |
| AAT-22841 | Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Green Fluorescence Optimized for Flow Cytometry* | 100Tests |
| AAT-22842 | Cell Meter™ Fluorimetric Fixed Cell Cycle Assay Kit *Red Fluorescence Optimized for Flow Cytometry* | 100Tests |
| AAT-22843 | Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit II *Triple Fluorescence Colors* | 100Tests |
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文献和实验相关实验
领域奠基之作：Cell 重磅报道可胞外展示的糖基化修饰 RNA——glycoRNA
，利用叠氮化物基团的前体糖来标记细胞或动物，从而发现蛋白质相关多糖。在利用其标记唾液酸前体（Ac4ManNAz）时，发现标记细胞中高度纯化的 RNA 中存在叠氮化物反应，提示潜在的多糖化 RNA 存在。 为了探究唾液酸化多糖修饰的 RNA（glycoRNA）是否存在，研究人员利用 Ac4ManNAz 标记 HeLa 细胞，然后提纯高纯度的 RNA，随后通过变性凝胶电泳分离和印迹分析，发现标记信号是时间依赖的，并且 DNase 处理并不影响 glycoRNA 信号，而 RNase 处理的影响可被抑制
非皂化脂质。是广泛地分布于动植物中的黄、橙、红或紫色的一组色素。构成发色原因的共轭二重键具有长链聚烯烃结构。通常是几种混在一起生成。具有C40 的萜结构的较多，不含氮。已知天然类胡萝卜素约有 300种，其中不含氧的碳化氢类有胡萝卜素、菌脂素等；含氧的非常多，有醇、酮、醚、醛、环氧化物、羰酸和酯等。它们之中大量存在的有岩藻黄质（ fucoxanthin）、叶黄素（ lutein）、堇菜黄质（ violaxanthin）、新黄质（ neoxanthin）等，均属于胡萝卜醇。类胡萝卜素多数
，这也是低温保鲜的原因之一。 (3)营养元素 叶绿素的形成必须有一定的营养元素。氮和镁是叶绿素的组成成分，铁、锰、铜、锌等则在叶绿素的生物合成过程中有催化功能或其它间接作用。因此，缺少这些元素时都会引起缺绿症(chlorosis)，其中尤以氮的影响最大，因而叶色的深浅可作为衡量植株体内氮素水平高低的标志。 (4)氧 缺氧能引起Mg-原卟啉IX或Mg-原卟啉甲酯的积累，影响叶绿素的合成。 (5)水 缺水不但影响叶绿素生物合成，而且还促使原有叶绿素加速分解，所以干旱时叶片呈黄褐色。
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一氧hua氮NO荧光探针 DAX-J2 橙
￥1836
