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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 保质期:
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- 英文名:
2-(4-Bromophenyl)-5-phenylthiophene
- 库存:
咨询
- 供应商:
无锡云萃生物
- CAS号:
118621-30-0
- 规格:
2mg/10mg/500mg/500g
| 规格: | 2mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
| 规格: | 500g | 产品价格: | ¥100.0 |
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文献和实验deionized formamide 500 m l 10X TBE 500 m l 0.2% bromophenyl blue and 0.2% xylene cyanol Other Reagents Needed: 0.22 m M disposable syringe filters, short wave UV source, intensifying screen for UV shadowing Procedure • Pour a 20% denaturing gel: 12 ml
In vitro transcription with yeast nuclear extract
if desired but is not necessary) 10 units RNAse inhibitor (Amersham ~105 units/microliter) 24 ng Gal4-VP16 (0.2 microliters 0.12 mg/ml) or 30 ng Gal4-AH (0.3 microliters 0.1 mg/ml) H20 to a final volume of 20 microliters (the final
Steve HahnLast modified10/16/98Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed mutagenesis from around 30% to ~75% or greater in some cases .1. Use PAGE










