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- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Annexin V, FITC Labeled
- CAS号：
/
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
100 tests
关键词：Annexin V, FITC标记详细如下
Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This fluorescent FITC-Annexin V conjugate is widely used for monitoring cell apoptosis with a flow cytometer or a fluorescence microscope. For microplate assays of annexin V-based cell apoptosis detection, we recommend you use our Cell Meter™ kits.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
| 货号 | 英文名称 | 规格 |
| AAT-110501K0 | PE Anti-mouse CD105 Antibody *MJ7/18* | 25tests |
| AAT-110501K1 | PE Anti-mouse CD105 Antibody *MJ7/18* | 100tests |
| AAT-110501K2 | PE Anti-mouse CD105 Antibody *MJ7/18* | 500tests |
| AAT-110501L0 | PE/Cy5 Anti-mouse CD105 Antibody *MJ7/18* | 25tests |
| AAT-110501L1 | PE/Cy5 Anti-mouse CD105 Antibody *MJ7/18* | 100tests |
| AAT-110501L2 | PE/Cy5 Anti-mouse CD105 Antibody *MJ7/18* | 500tests |
| AAT-110501M0 | PE/Cy7 Anti-mouse CD105 Antibody *MJ7/18* | 25tests |
| AAT-110501M1 | PE/Cy7 Anti-mouse CD105 Antibody *MJ7/18* | 100tests |
| AAT-110501M2 | PE/Cy7 Anti-mouse CD105 Antibody *MJ7/18* | 500tests |
| AAT-110501N0 | PE/AF610 Anti-mouse CD105 Antibody *MJ7/18* | 25tests |
| AAT-110501N1 | PE/AF610 Anti-mouse CD105 Antibody *MJ7/18* | 100tests |
| AAT-110501N2 | PE/AF610 Anti-mouse CD105 Antibody *MJ7/18* | 500tests |
| AAT-110501O0 | PE/AF700 Anti-mouse CD105 Antibody *MJ7/18* | 25tests |
| AAT-110501O1 | PE/AF700 Anti-mouse CD105 Antibody *MJ7/18* | 100tests |
| AAT-110501O2 | PE/AF700 Anti-mouse CD105 Antibody *MJ7/18* | 500tests |
| AAT-110501P0 | PE/iFluor™ 647 Anti-mouse CD105 Antibody *MJ7/18* | 25tests |
| AAT-110501P1 | PE/iFluor™ 647 Anti-mouse CD105 Antibody *MJ7/18* | 100tests |
| AAT-110501P2 | PE/iFluor™ 647 Anti-mouse CD105 Antibody *MJ7/18* | 500tests |
| AAT-110501Q0 | PE/iFluor™ 750 Anti-mouse CD105 Antibody *MJ7/18* | 25tests |
| AAT-110501Q1 | PE/iFluor™ 750 Anti-mouse CD105 Antibody *MJ7/18* | 100tests |
| AAT-110501Q2 | PE/iFluor™ 750 Anti-mouse CD105 Antibody *MJ7/18* | 500tests |
| AAT-110501R0 | PE/Texas Red® Anti-mouse CD105 Antibody *MJ7/18* | 25tests |
| AAT-110501R1 | PE/Texas Red® Anti-mouse CD105 Antibody *MJ7/18* | 100tests |
| AAT-110501R2 | PE/Texas Red® Anti-mouse CD105 Antibody *MJ7/18* | 500tests |
| AAT-110501S0 | PerCP Anti-mouse CD105 Antibody *MJ7/18* | 25tests |
| AAT-110501S1 | PerCP Anti-mouse CD105 Antibody *MJ7/18* | 100tests |
| AAT-110501S2 | PerCP Anti-mouse CD105 Antibody *MJ7/18* | 500tests |
| AAT-110501T0 | PerCP/Cy5.5 Anti-mouse CD105 Antibody *MJ7/18* | 25tests |
| AAT-110501T1 | PerCP/Cy5.5 Anti-mouse CD105 Antibody *MJ7/18* | 100tests |
| AAT-110501T2 | PerCP/Cy5.5 Anti-mouse CD105 Antibody *MJ7/18* | 500tests |
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文献和实验Q：Annexin V 凋亡检测试剂盒的原理是什么？ A：Annexin V 是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸 PS 有高度亲和力，可通过细胞外侧暴露的 PS 与凋亡早期细胞胞膜结合，将 Annexin V 标记荧光染料如 FITC 利用流式细胞仪或荧光显微镜可检测细胞凋亡。 碘化丙啶(Propidium Iodide, PI)或 7-AAD 可与双链 DNA 特异性结合，并产生强烈的荧光，正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性，核染料 PI 或 7-AAD
磷脂酰丝氨酸（Phosphatidylserine, PS）正常位于细胞膜的内侧，但在细胞凋亡的早期，PS可从细胞膜的内侧翻转到细胞膜的表面，暴露在细胞外环境中（图3）。Annexin-V是一种分子量为35~36KD的Ca2+依赖性磷脂结合蛋白，能与PS高亲和力特异性结合。将Annexin-V进行荧光素（FITC、PE）或biotin标记，以标记了的Annexin-V作为荧光探针，利用流式细胞仪或荧光显微镜可检测细胞凋亡的发生。碘化丙啶（propidine iodide, PI）是一种核酸
示例： Jurkat细胞用1μM喜树碱(Camptothecin) (左)或未加药 (右)处理4h，FITC-Annexin V/PI荧光双染细胞凋亡检测试剂盒染色后，流式细胞仪荧光检测。 Annexin V-FITC单阳性细胞为早期凋亡细胞，Annexin V-FITC和PI染色双阳性的细胞为坏死细胞或者晚期凋亡。PI单染色阳性位裸核细胞。 实测数据可能会因细胞类型、细胞凋亡情况，检测仪器等的不同而存在差异，图中数据仅供参考。 流式检测细胞凋亡的数据分析方法： 1） 选中所有颗粒，将FSC
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