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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 英文名:
2-(2-Nitroanilino)ethanol
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
4926-55-0
- 规格:
2mg/10mg/500mg/500g
| 规格: | 2mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
| 规格: | 500g | 产品价格: | ¥100.0 |
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文献和实验DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
volume, so adjust as necessary). Incubate overnight at 55 C. Day 4: Remove all parafilm from tubes. Inactivate Proteinase K for 10-15 minutes at 95 C in PCR machine or hot water bath. Remove oil by rolling samples and oil on parafilm and and pipette
a. Pool fractions with an A260 of 1.00 or greater into 35 ml Corex glass tubes, add one volume of ddH2O, and ethanol precipitate by adding 2.5 volumes of cold 95% ethanol. Incubate at least 2 hours at -20degC, centrifuge at 10,000 rpm for 45 minutes
Technologies) 100% ethanol 2X loading dye (95% formamide, 20 mM EDTA, 0.05% bromophenol blue, 0.05% xylene cyanol) 2. Methods (Under sterile, RNase free conditions) Note: The protocol was utilized to screen for tumor-specific alterations






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