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文献和实验MicroRNA和siRNA克隆规程（MicroRNA and siRNA Cloning Protocol）
or with automated algorithms OLIGOS FOR SMALL RNA CLONING Underline nucleotides mark a Ban I restriction digest site (G↓GYRCC) 3" End Donor Oligo (available from IDT Inc. as the miRNA cloning linker) App17.91x
Quantitative Stem-Loop RT-PCR for Detection of MicroRNAs
and reliable manner. First, a miRNA-specific stem-loop RT primer is hybridized to the miRNA and then reverse transcribed. Next, the RT product is amplified and monitored in real time using a miRNA-specific forward primer and the universal reverse primer
DOP-PCR Amplification of Whole Genomic DNA and Microchip-Based Capillary Electrophoresis
). In a more demanding protocol, called linker adaptor-PCR, RsaI restricted genomic DNA fragments are ligated to SmaI-cut pUC plasmid. Subsequently, the inserts are amplified by PCR using the universal M13/pUC sequencing and reverse sequencing
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