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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
BrdU [5-Bromo-2'-deoxyuridine] *CAS 59-14-3*
- CAS号：
59-14-3
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
25 mg
关键词：BrdU 细胞增殖检测试剂 CAS 59-14-3详细如下
5-Bromo-2-deoxyuridine (BrdU) is an analog of thymidine, and can be incorporated into the newly synthesized DNA of replicating cells (S-phase cells). BrdU is a commonly used in the detection of proliferating cells in living tissues. It works by substituting for thymidine during DNA replication and incorporating itself into the newly synthesized DNA (during S-phase). Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Because BrdU can replace thymidine during the S phase of cell division, it can cause mutations.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
| 货号 | 英文名称 | 规格 |
| AAT-102900O0 | iFluor™ 810 Anti-human CD29 Antibody *HI29a* | 100tests |
| AAT-102900O1 | iFluor™ 810 Anti-human CD29 Antibody *HI29a* | 500tests |
| AAT-102900P0 | iFluor™ 820 Anti-human CD29 Antibody *HI29a* | 100tests |
| AAT-102900P1 | iFluor™ 820 Anti-human CD29 Antibody *HI29a* | 500tests |
| AAT-102900Q0 | iFluor™ 840 Anti-human CD29 Antibody *HI29a* | 100tests |
| AAT-102900Q1 | iFluor™ 840 Anti-human CD29 Antibody *HI29a* | 500tests |
| AAT-102900R0 | iFluor™ 860 Anti-human CD29 Antibody *HI29a* | 100tests |
| AAT-102900R1 | iFluor™ 860 Anti-human CD29 Antibody *HI29a* | 500tests |
| AAT-102900S0 | iFluor™ A7 Anti-human CD29 Antibody *HI29a* | 100tests |
| AAT-102900S1 | iFluor™ A7 Anti-human CD29 Antibody *HI29a* | 500tests |
| AAT-102900T0 | mFluor™ Blue 570 Anti-human CD29 Antibody *HI29a* | 100tests |
| AAT-102900T1 | mFluor™ Blue 570 Anti-human CD29 Antibody *HI29a* | 500tests |
| AAT-102900U0 | mFluor™ Green 620 Anti-human CD29 Antibody *HI29a* | 100tests |
| AAT-102900U1 | mFluor™ Green 620 Anti-human CD29 Antibody *HI29a* | 500tests |
| AAT-102900V0 | mFluor™ Red 700 Anti-human CD29 Antibody *HI29a* | 100tests |
| AAT-102900V1 | mFluor™ Red 700 Anti-human CD29 Antibody *HI29a* | 500tests |
| AAT-102900W0 | mFluor™ Red 780 Anti-human CD29 Antibody *HI29a* | 100tests |
| AAT-102900W1 | mFluor™ Red 780 Anti-human CD29 Antibody *HI29a* | 500tests |
| AAT-102900X0 | mFluor™ UV375 Anti-human CD29 Antibody *HI29a* | 100tests |
| AAT-102900X1 | mFluor™ UV375 Anti-human CD29 Antibody *HI29a* | 500tests |
| AAT-102900Y0 | mFluor™ UV460 Anti-human CD29 Antibody *HI29a* | 100tests |
| AAT-102900Y1 | mFluor™ UV460 Anti-human CD29 Antibody *HI29a* | 500tests |
| AAT-102900Z0 | mFluor™ Violet 450 Anti-human CD29 Antibody *HI29a* | 100tests |
| AAT-102900Z1 | mFluor™ Violet 450 Anti-human CD29 Antibody *HI29a* | 500tests |
| AAT-10290100 | mFluor™ Violet 500 Anti-human CD29 Antibody *HI29a* | 100tests |
| AAT-10290101 | mFluor™ Violet 500 Anti-human CD29 Antibody *HI29a* | 500tests |
| AAT-10290110 | mFluor™ Violet 510 Anti-human CD29 Antibody *HI29a* | 100tests |
| AAT-10290111 | mFluor™ Violet 510 Anti-human CD29 Antibody *HI29a* | 500tests |
| AAT-10290120 | mFluor™ Violet 540 Anti-human CD29 Antibody *HI29a* | 100tests |
| AAT-10290121 | mFluor™ Violet 540 Anti-human CD29 Antibody *HI29a* | 500tests |
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文献和实验willion1985 直接测定DNA合成是细胞增殖检测的最准确方法之一，是测定物质毒性、评估药物安全评价、细胞健康的基本方法，其中以前常用的方式是利用胸腺嘧啶核苷酸类似物----BrdU进行检测。因为在细胞周期的S期，和细胞一起孵育的BrdU能渗入DNA分子中，再结合BrdU抗体与渗入DNA的BrdU特异性结合，就能够检测到DNA复制活跃的细胞。 但BrdU有一大缺点，就是需要变性DNA后才能与抗体结合，但这就破坏了DNA双链结构，影响了其他染料的结合
5-brdu 的分子量为307.1，将100mg分为三份30.7mg（0.1mmol），溶于1ml三蒸水，分装-20度保存。用的时候再稀释100倍，如在1ml溶液里加入10ul即可。尽量分装为小剂量，如100ul，避免反复冻融使其活性降低。1、BudR贮存液的配制BudR 5mg（先用0.5ml 1N NaOH溶解）然后加蒸馏水至5ml，即成1000ug/ml的贮存液，因BudR遇光会发生分解，贮存液需置棕色瓶并黑纸封瓶避光冰冻保存。 一般用的是用10μg/ml终浓度。2、终止细胞培养前，加入
质量不一致，造成难以确保实验重复性，且容易产生假阳性结果。 图1 BrdU需要DNA变性，但变性过分或没有变性都可能容易导致BrdU结果假阳性 与BrdU方法相比，EdU检测方法无需DNA变性，无需抗原修复，无需抗原抗体反应，更简单、更灵敏、更快速、更准确，是细胞增殖检测的最佳选择。 图2 BrdU与EdU检测原理示意图 表1 BrdU与EdU检测优缺点比较 更准确--无需DNA变性(酸解、热解、酶解等)，可有效避免
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