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- 库存：
大量
- 英文名：
Helixyte™ Green Fluorimetric dsDNA Quantitation Kit
- CAS号：
/
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
200 Tests
关键词：Helixyte Green 双链DNA荧光定量试剂盒 适合酶标仪检测检测详细如下
Helixyte™ Green dsDNA Quantitation Assay Kit can be used for selectively detecting as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides. Helixyte™ Green exhibits large fluorescence enhancement upon binding to dsDNA. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products. Helixyte™ Green dsDNA Quantitation Assay Kit is a few magnitudes more sensitive than UV absorbance readings. It is specific for dsDNA in the presence of equimolar amounts of RNA. The kit is robust with a mix and read format. It can be used with a bench top fluorometer or a hand-held fluorescence meter (e.g., Qubit fluorometer). This kit is an excellent replacement for Quant-iT™ PicoGreen® dsDNA Assay Kit (Quant-iT™ and PicoGreen® are the trademarks of Invitrogen).
Components
Component A: Helixyte Green™ 1 vial (1 mL, 200X in DMSO)
Component B: 20X Assay Buffer 1 bottle (25 mL)
Component C: Calf thymus DNA Standard 1 vial (1 mL, 100 µg/mL)
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
| 货号 | 英文名称 | 规格 |
| AAT-102400Z0 | mFluor™ Violet 450 Anti-human CD24 Antibody *HI45* | 100tests |
| AAT-102400Z1 | mFluor™ Violet 450 Anti-human CD24 Antibody *HI45* | 500tests |
| AAT-10240100 | mFluor™ Violet 500 Anti-human CD24 Antibody *HI45* | 100tests |
| AAT-10240101 | mFluor™ Violet 500 Anti-human CD24 Antibody *HI45* | 500tests |
| AAT-10240110 | mFluor™ Violet 510 Anti-human CD24 Antibody *HI45* | 100tests |
| AAT-10240111 | mFluor™ Violet 510 Anti-human CD24 Antibody *HI45* | 500tests |
| AAT-10240120 | mFluor™ Violet 540 Anti-human CD24 Antibody *HI45* | 100tests |
| AAT-10240121 | mFluor™ Violet 540 Anti-human CD24 Antibody *HI45* | 500tests |
| AAT-10240140 | AF350 Anti-human CD24 Antibody *HI45* | 100tests |
| AAT-10240141 | AF350 Anti-human CD24 Antibody *HI45* | 500tests |
| AAT-10240150 | AF488 Anti-human CD24 Antibody *HI45* | 100tests |
| AAT-10240151 | AF488 Anti-human CD24 Antibody *HI45* | 500tests |
| AAT-10240160 | AF555 Anti-human CD24 Antibody *HI45* | 100tests |
| AAT-10240161 | AF555 Anti-human CD24 Antibody *HI45* | 500tests |
| AAT-10240170 | AF594 Anti-human CD24 Antibody *HI45* | 100tests |
| AAT-10240171 | AF594 Anti-human CD24 Antibody *HI45* | 500tests |
| AAT-10240180 | AF647 Anti-human CD24 Antibody *HI45* | 100tests |
| AAT-10240181 | AF647 Anti-human CD24 Antibody *HI45* | 500tests |
| AAT-10240190 | AF680 Anti-human CD24 Antibody *HI45* | 100tests |
| AAT-10240191 | AF680 Anti-human CD24 Antibody *HI45* | 500tests |
| AAT-102401A0 | AF700 Anti-human CD24 Antibody *HI45* | 100tests |
| AAT-102401A1 | AF700 Anti-human CD24 Antibody *HI45* | 500tests |
| AAT-102401B0 | AF750 Anti-human CD24 Antibody *HI45* | 100tests |
| AAT-102401B1 | AF750 Anti-human CD24 Antibody *HI45* | 500tests |
| AAT-102401C0 | APC Anti-human CD24 Antibody *HI45* | 25tests |
| AAT-102401C1 | APC Anti-human CD24 Antibody *HI45* | 100tests |
| AAT-102401C2 | APC Anti-human CD24 Antibody *HI45* | 500tests |
| AAT-102401D0 | APC/Cy7 Anti-human CD24 Antibody *HI45* | 25tests |
| AAT-102401D1 | APC/Cy7 Anti-human CD24 Antibody *HI45* | 100tests |
| AAT-102401D2 | APC/Cy7 Anti-human CD24 Antibody *HI45* | 500tests |
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文献和实验地点：中国农科院重大工程楼 107 为检验BioTeke公司实时荧光定量试剂（SYBR GreenⅠ）质量，本次实验分别采用国际知名的ABI公司（ABI Power SYBR Green PCR Master Mix）、TOYOBO公司（Realtime SYBR GreenⅠPCR Master Mix）和BioTeke公司产品进行实时荧光定量PCR分析，实验过程中除了荧光定量试剂Mix不同，其它反应条件（模板、引物和反应
了长足的发展，比如BioTeke公司的荧光定量系列产品就以其优良的品质和亲民的价格越来越受到国内用户的青睐，目前还可以申请试用装。为了证明其荧光定量系列产品的品质，BioTeke公司特邀请了山东大学细胞发育遗传学实验室进行了BioTeke的SYBR GREEN I实时荧光定量产品和T公司的实时荧光定量试剂的比对实验。具体实验如下：实验材料小鼠肝脏组织山东大学实验室提供样品cDNA实验方法RNA提取：Bioteke RNApure高纯总RNA提取试剂盒，操作步骤请参考说明书。目的基因：小鼠管家基因β–
分析.4、几个概念：（1）扩增曲线 ：（2） 荧光阈值：（3）Ct值：CT值的重现性：5、定量原理：理想的PCR反应： X=X0*2n非理想的PCR反应： X=X0 (1+Ex)nn：扩增反应的循环次数X：第n次循环后的产物量X0：初始模板量Ex：扩增效率5、标准曲线6、绝对定量1）确定未知样品的 C(t)值2）通过标准曲线由未知样品的C(t)值推算出其初始量二、实时荧光定量PCR的几种方法介绍 方法一：SYBR Green法 （一）工作原理 1、SYBR Green 能结合到双链DNA的小沟
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