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- 库存：
大量
- 英文名：
Cell Meter™ Intracellular NADH/NADPH Fluorescence Imaging Kit
- CAS号：
/
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
100 Tests
关键词：Cell Meter 胞内NADH / NADPH荧光成像分析试剂盒详细如下
The detection of intracellular dihydronicotinamide adenine dinucleotide NADH and its phosphate ester NADPH is important for disease diagnostics and drug discovery. In general, the redox couples NAD/NADH and NADP/NADPH play a critical role in energy metabolism, glycolysis, tricarboxylic acid cycle and mitochondrial respiration. The increased NAD(P)H level in cells is linked to the abnormal production of reactive oxygen species (ROS) and DNA damage. However, due to the lack of sensitive NAD(P)H probe, it has been challenging to detect intracellular NAD(P)H in biological systems. Cell Meter™ Intracellular NADH/NADPH Fluorescence Imaging Kit provides an efficient method to monitor intracellular NAD(P)H level in live cells. JZL1707 NAD(P)H sensor has been developed as an excellent fluorescent probe for detecting and imaging NADH/NADPH in cells. The probe bind NADH/NADPH to generate strong fluorescence signal with high sensitivity and specificity. JZL1707 NAD(P)H sensor can be readily loaded into live cells, and its fluorescence signal can be conveniently monitored using the filter set of Cy3® or TRITC. This kit is optimized for fluorescence imaging and microplate reader applications.
Components
Component A: JZL1707 NAD(P)H Sensor 1 vial (40 µL)
Component B: Assay Buffer 1 bottle (20 mL)
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
| 货号 | 英文名称 | 规格 |
| AAT-100320M0 | iFluor™ 790 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100320M1 | iFluor™ 790 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100320N0 | iFluor™ 800 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100320N1 | iFluor™ 800 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100320O0 | iFluor™ 810 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100320O1 | iFluor™ 810 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100320P0 | iFluor™ 820 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100320P1 | iFluor™ 820 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100320Q0 | iFluor™ 840 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100320Q1 | iFluor™ 840 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100320R0 | iFluor™ 860 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100320R1 | iFluor™ 860 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100320S0 | iFluor™ A7 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100320S1 | iFluor™ A7 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100320T0 | mFluor™ Blue 570 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100320T1 | mFluor™ Blue 570 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100320U0 | mFluor™ Green 620 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100320U1 | mFluor™ Green 620 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100320V0 | mFluor™ Red 700 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100320V1 | mFluor™ Red 700 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100320W0 | mFluor™ Red 780 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100320W1 | mFluor™ Red 780 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100320X0 | mFluor™ UV375 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100320X1 | mFluor™ UV375 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100320Y0 | mFluor™ UV460 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100320Y1 | mFluor™ UV460 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100320Z0 | mFluor™ Violet 450 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100320Z1 | mFluor™ Violet 450 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-10032100 | mFluor™ Violet 500 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-10032101 | mFluor™ Violet 500 Anti-human CD3 Antibody *UCHT1* | 500tests |
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文献和实验，第四个结果就有用ELISA检测胞内VEGF的报道。 http://www.nature.com/leu/journal/v17/n10/full/2403084a.html For intracellular levels of VEGF, 5x 106 cells from each cell line were washed in RPMI x2, snap frozen in liquid nitrogen, and then thawed at 37°C
、溶酶体与疾病 各类贮积症 台-萨氏综合征（Tay-Sachs diesease）：溶酶体缺少氨基已糖酯酶A，导致神经节甘脂GM2积累。 II型糖原累积病（Pompe病）：缺乏α-1,4-葡萄糖苷酶，糖原在溶酶体中积累。 Gaucher病：缺乏β- 葡萄糖苷酶，葡糖脑苷脂沉积。 细胞内含物病（inclusion-cell disease）：N-乙酰葡糖胺磷酸转移酶单基因突变。高尔基体中加工的溶酶体前酶上不能形成M6P分选信号，病人成纤维细胞的溶酶体中没有水解酶，底物在溶酶体中贮积，形成
The MTT Cell Proliferation Assay
proliferation. The yellow tetrazolium MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) is reduced by metabolically active cells, in part by the action of dehydrogenase enzymes, to generate reducing equivalents such as NADH and NADPH
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