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48T
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Japanese.
Characteristics: Can be induced to differentiate into megakaryocytes after application of 12-O-tetradecanoyl-13-phorbol acetate (TPA) or valproic acid (VPA) (PubMed=1742484; PubMed=30332548).
Doubling time: 36-48 hours (PubMed=2998511); ~35 hours (DSMZ=ACC-364).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Misspelling: Mego-1; Note=Occasionally.
Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371.
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文献和实验*发表【中文论文】请标注:由上海酶研生物科技有限公司提供;
*发表【英文论文】请标注:From Shanghai EK-Bioscience Biotechnology Co., Ltd.
-1640/10%FCS Anti-human CD95, clone EOS9.1 (eBioscience Cat. No. 16-0958) 24-well culture plate (Costar Cat. No. 3526) 12x75 mm flow cytometry centrifuge tubes (Falcon Cat. No. 2008) Apoptosis detection kit (eBioscience Apo-Direct
FACS Procedures for Apoptosis Detection
7-AAD for the PI provided with the kit. Materials: Apoptosis Detection Kit (R&D Systems KNX50) . contains: FITC-conjugated human annexin V, PI, 10x Binding Buffer. 7-Amino-actinomycin (Sigma A-9400)
Guide to Cell Proliferation and Apoptosis Methods
Kit 11 Cell Death Detection ELISAPLUS 13 1.2.1.2 Assays that measure apoptosis-induced proteases (caspase) 16 Caspase
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