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文献和实验Human Myoblasts and Muscle-Derived SP Cells
have demonstrated hematopoietic and myogenic differentiation potential both in vitro and in vivo (11 -14 ) and these studies are being extended to human-derived SP cells. Methods to isolate human muscle SP cells from fetal and from adult skeletal muscle
: 12,500 rpm for 15' at 4 deg.C in HB-4. Post fix in methanol (-20 deg.C) for 5' and rehydrate and do 5051 + antitubulin followed by anti-mouse, anti-human secondaries. Assess peak by concentration of double staining dots, pool and freeze in liq N2
Infection of Human Monocyte-Derived Macrophages With Coxiella burnetii
from cell culture and the use of these preparations to infect primary macrophages derived from human peripheral blood monocytes. Both virulent phase I and avirulent phase II C. burnetii productively infect human monocyte-derived macrophages (MDMs
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