RANKL - Mouse RANKL protein

产品详情：重组小鼠 RANKL 蛋白 (无标签)

货号：PRP1114

产品类型：细胞因子

种属来源：小鼠

产品概述

本产品为高纯度、无标签的重组小鼠 RANKL (Receptor Activator of NF-κB Ligand) 蛋白。该蛋白是通过大肠杆菌表达系统制备的冻干粉，氨基酸序列来源于小鼠 RANKL (UniProt: O35235) 的 Pro143-Asp316 片段。其预测分子量为 19 kDa，在还原性 SDS-PAGE 中表观分子量约为 19 kDa。本品经严格质控，纯度经 SDS-PAGE 检测 ≥ 98%，且内毒素 (LAL 法) 水平低，每 1μg 蛋白质中 EU 含量 < 1.0，旨在为您的细胞培养与研究应用提供可靠的支持。

关键特性

• 高纯度：经 SDS-PAGE 检测，纯度 ≥ 98%
• 无标签：尽可能减少了因标签干扰实验结果的潜在风险
• 生物活性已验证：通过功能性 ELISA 检测其与受体 OPG 的结合能力，人 OPG 的 EC50 值为 55 ng/mL
• 低内毒素：LAL 法检测，每 1μg 蛋白质中 EU 含量 < 1.0
• 应用广泛：适用于多种细胞生物学研究，特别是在破骨细胞分化、免疫调节等研究领域

产品信息

表达宿主	大肠杆菌
蛋白长度	174个氨基酸 (N-Met)
预测分子量	19 kDa
表观分子量	~19 kDa (还原性 SDS-PAGE)
产品形式	冻干粉
冻干缓冲液	无菌 PBS, pH 7.4

活性检测

通过功能性 ELISA 方法检测其生物活性。包被 1 μg/mL (100μl/孔) 的小鼠 sRANKL 可有效结合人 OPG (C-Fc)，计算得出人 OPG 的 EC50 值为 55 ng/mL。

使用注意事项

• 开盖前请先离心，以避免粉末损失。
• 推荐使用产品配套的复溶缓冲液进行复溶，复溶浓度建议不低于 100 µg/mL。
• 复溶后可根据具体的实验需求进行进一步稀释。
• 复溶后的蛋白溶液可在 4℃ 下短期保存 2-7 天。
• 如需长期保存，建议添加载体蛋白 (如 0.1% HSA 或 BSA)，分装后置于 -20℃ 以下环境，并避免反复冻融。

保存与运输

• 保存建议：冻干粉应保存于 -20℃ 以下的干燥环境中。复溶后，若需长期保存请置于 -20℃ 以下。
• 运输条件：常温运输。

背景介绍

RANKL (TNFSF11) 是肿瘤坏死因子 (TNF) 配体超家族成员，作为核因子 κB 受体激活因子 (RANK) 的配体，在免疫调节、破骨细胞分化及骨吸收等关键生物学过程中扮演着核心角色。它由成骨细胞、基质细胞和活化的 T 淋巴细胞等多种细胞表达，通过与受体 RANK 结合或与诱骗受体 OPG (骨保护素) 相互作用，精密调控树突状细胞存活、T 细胞激活以及骨代谢平衡。

规格与价格

规格	价格 (人民币)	货期
5 μg	588	现货 (次日发货)
20 μg	1498	请咨询
100 μg	4698	现货 (次日发货)
1 mg	请咨询	请咨询

警告：本品仅供研究使用，不适用于人类或临床诊断。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Details: Recombinant Mouse RANKL Protein (Tag-Free)

Catalog Number: PRP1114

Product Type: Cytokine

Species: Mouse

Product Overview

This product is a high-purity, tag-free recombinant mouse RANKL (Receptor Activator of NF-κB Ligand) protein. It is produced as a lyophilized powder using an E. coli expression system and corresponds to amino acids Pro143-Asp316 of mouse RANKL (UniProt: O35235). The predicted molecular weight is 19 kDa, and the apparent molecular weight is approximately 19 kDa as determined by reducing SDS-PAGE. Rigorous quality control confirms a purity of ≥98% by SDS-PAGE and low endotoxin levels (<1.0 EU/μg protein, LAL method), ensuring reliable support for your cell culture and research applications.

Key Features

• High Purity: ≥98% purity as determined by SDS-PAGE.
• Tag-Free: Minimizes potential interference from tags in experimental outcomes.
• Biologically Active: Binding activity to its receptor OPG verified by functional ELISA, with an EC₅₀ value of 55 ng/mL for human OPG.
• Low Endotoxin: <1.0 EU/μg protein as determined by the LAL method.
• Broad Applications: Suitable for various cell biology studies, particularly in osteoclast differentiation, immunoregulation, and related research fields.

Product Information

Expression Host	E. coli
Protein Length	174 amino acids (N-Met)
Predicted Molecular Weight	19 kDa
Apparent Molecular Weight	~19 kDa (Reducing SDS-PAGE)
Form	Lyophilized powder
Lyophilization Buffer	Sterile PBS, pH 7.4

Activity Assay

Biological activity was measured by functional ELISA. Coating with 1 μg/mL (100μl/well) of mouse sRANKL effectively binds human OPG (C-Fc), yielding an EC₅₀ value of 55 ng/mL for human OPG.

Handling Instructions

• Centrifuge the vial before opening to avoid powder loss.
• Reconstitution using the provided buffer is recommended to a concentration not less than 100 μg/mL.
• Further dilution can be performed according to specific experimental requirements after reconstitution.
• The reconstituted protein solution can be stored at 4°C for short-term use (2-7 days).
• For long-term storage, it is advisable to add a carrier protein (e.g., 0.1% HSA or BSA), aliquot, and store below -20°C, avoiding repeated freeze-thaw cycles.

Storage and Shipping

• Storage: Lyophilized powder should be stored dry below -20°C. After reconstitution, for long-term storage, store below -20°C.
• Shipping: Ambient temperature.

Background

RANKL (TNFSF11), a member of the tumor necrosis factor (TNF) ligand superfamily, acts as the ligand for the receptor activator of nuclear factor κB (RANK). It plays a central role in key biological processes such as immunoregulation, osteoclast differentiation, and bone resorption. Expressed by various cells including osteoblasts, stromal cells, and activated T lymphocytes, RANKL precisely regulates dendritic cell survival, T cell activation, and bone metabolism balance through binding to its receptor RANK or interacting with the decoy receptor OPG (osteoprotegerin).

Specifications & Pricing

Size	Price (RMB)	Availability
5 μg	588	In Stock (Ships Next Day)
20 μg	1498	Please Inquire
100 μg	4698	In Stock (Ships Next Day)
1 mg	Please Inquire	Please Inquire

Warning: This product is for research use only. It is not intended for human or diagnostic use.

Note: The above information is subject to change due to product optimization and upgrades. Please contact YaKeyin Company for specific details.