IL-28B - Human IL-28B Protein,

Human IL-28B Protein, His tag (Animal-Free)

货号: PRP1153 | 产品类型: 重组细胞因子

产品概述

本品为高纯度、动物源无成分（Animal-Free）的重组人白细胞介素28B（IL-28B）蛋白，C端带有His标签，通过大肠杆菌表达系统制备。该蛋白具有显著的抗病毒与免疫调节活性，适用于细胞培养及相关生物医学研究。

关键特性

• 高纯度: 经SDS-PAGE验证，纯度 > 95%
• 低内毒素: 每微克蛋白内毒素含量 < 0.01 EU（LAL法检测）
• 动物源无: 生产全程无动物源成分，降低外源因子风险
• 生物活性明确: 对HepG2细胞感染EMC病毒的保护作用ED₅₀ < 5 ng/mL

技术参数

使用说明

复溶建议: 推荐使用配套复溶缓冲液，浓度不低于100 µg/mL；后续可根据实验需求稀释。

保存条件: 冻干粉-20°C保存1年；复溶后2-8°C可保存1周；稀释后分装-20°C/-80°C可保存3-6个月，避免反复冻融。

注意事项: 使用前请离心；本品仅供研究使用。

背景信息

IL-28B（干扰素λ3，IFN-λ3）属于III型干扰素家族，分子量约19.77 kDa，由上皮细胞表达。其通过结合IFNLR1受体激活JAK/STAT信号通路，在抗病毒、抗肿瘤及免疫调节中发挥关键作用。

规格与价格

运输条件：冰袋运输（蓝冰）

警告: 本品仅用于科研，不适用于人体或临床诊断。使用前请查阅相关专利许可。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human IL-28B Protein, His tag (Animal-Free)

Catalog Number: PRP1153 | Product Type: Recombinant Cytokine

Product Overview

This product is a highly pure, Animal-Free recombinant human Interleukin-28B (IL-28B) protein with a C-terminal His tag, produced in an E. coli expression system. This protein exhibits significant antiviral and immunomodulatory activities and is suitable for cell culture and related biomedical research.

Key Features

• High Purity: > 95% purity as verified by SDS-PAGE
• Low Endotoxin: Endotoxin level < 0.01 EU per µg protein (LAL method)
• Animal-Free: Manufactured entirely without animal-derived components, reducing the risk of adventitious agents
• Well-Defined Bioactivity: ED₅₀ < 5 ng/mL for protective effect against EMC virus infection in HepG2 cells

Technical Parameters

Usage Instructions

Reconstitution Recommendation: Reconstitute to a concentration ≥ 100 µg/mL using the recommended reconstitution buffer; subsequent dilutions can be made based on experimental requirements.

Storage Conditions: Lyophilized powder stable for 1 year at -20°C; after reconstitution, store at 2-8°C for up to 1 week; for long-term storage, aliquot diluted product and store at -20°C/-80°C for 3-6 months. Avoid repeated freeze-thaw cycles.

Precautions: Centrifuge briefly before use. For research use only.

Background Information

IL-28B (Interferon Lambda 3, IFN-λ3) belongs to the type III interferon family, with a molecular weight of approximately 19.77 kDa, and is expressed by epithelial cells. It activates the JAK/STAT signaling pathway by binding to the IFNLR1 receptor, playing a key role in antiviral, antitumor, and immunomodulatory activities.

Specifications & Pricing

Shipping Conditions: Shipped with ice packs (blue ice)

Warning: This product is for research use only. Not intended for human or diagnostic use. Please review relevant patent licenses before use.

Note: The information provided above is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Arcano Company.