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- 详细信息
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- 保存条件:
详见说明
- 保质期:
详见说明
- 库存:
50株
- 供应商:
上海博尔森生物科技有限公司
- 规格:
10×100ul/50×100ul/100×100ul
| 规格: | 10×100ul | 产品价格: | ¥240.0 |
|---|---|---|---|
| 规格: | 50×100ul | 产品价格: | ¥1020.0 |
| 规格: | 100×100ul | 产品价格: | ¥1632.0 |
其中10种农杆菌化学转化感受态产品仅需冰、液氮和37℃ 热激操作即可,实验简单方便,经pCAMBIA2301 (11.6 Kb)质粒检测转化效率可达1×104 cfu/μg。同时提供7种农杆菌电击感受态产品,可成功转化大于50 Kb的载体,经pCAMBIA2301 (11.6 Kb)质粒检测转化效率可达1×105 cfu/μg。
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文献和实验Preparing chemically competent cells
Materials Plate of cells to be made competent TSS buffer LB media Ice Glassware & Equipment Falcon tubes 500μl Eppendorf tubes, on ice 200ml conical flask 200μl pipetman or repeating
TOP10 chemically competent cells
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free for these protocols. The easiest way to do this is to avoid washing glassware, and simply rinse it out. Autoclaving glassware filled
CCMB buffer to make chemical competent cell
TOP10 chemically competent cells ( modified from http://openwetware.org/wiki/TOP10_chemically_competent_cells#CCMB80_buffer) I also use this protocol to make Ecoli compent cell. It is pretty good. You can start from the fresh clone on plate
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