快速蛋白质定量试剂盒（BCA法）

快速蛋白质定量试剂盒（BCA法）- KTD3010

产品中心：细胞分析 | 产品分类：爆款产品 | 货号：KTD3010

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核心应用场景

专为蛋白类样本设计，适用于细胞器提取和染色实验中的蛋白质浓度测定，兼容生物医学研究与体外分析场景。

试剂盒组分

• 试剂A（稳定型螯合剂）
• 试剂B（显色增强剂）
• BSA标准品（预稀释梯度）

技术优势解析

• 5分钟显色技术：室温反应即达显色终点，较传统BCA法提速4倍
• 超宽检测窗口：1-2000 µg/mL线性范围，覆盖常规样本至微量样本检测
• 突破性抗干扰：耐受5% SDS/Triton X-100/Tween系列去污剂
• 微量样本适配：仅需1-20 µL待测液，节约珍贵样本

关键检测参数

最佳检测波长	480 nm
灵敏度阈值	1 µg/mL
显色稳定性	＞2小时（室温避光）

操作注意事项

1. 需规避还原剂干扰：样本中DTT/β-巯基乙醇含量需＜1 mM
2. 建议同步标准曲线：每批次实验需建立独立标准曲线
3. 高背景样本处理：推荐改用Bradford法试剂盒（货号KTD3002）

▍ 存储与物流

• 保存条件：组分分装避光保存，有效期12个月
• 运输方案：蓝冰低温运输，保障试剂稳定性

⚠ 重要声明

本产品仅限科研用途，不可用于临床诊断。实验操作需遵守当地生物安全法规，使用前请充分验证实验方案合规性。

技术背景

基于改良螯合剂技术，通过Cu⁺特异性络合反应实现快速显色。在碱性环境中，蛋白分子将Cu²⁺还原为Cu⁺，与螯合剂形成480 nm特征吸收峰复合物，突破传统BCA法的热稳定性限制。

规格与交付

500T规格 | 科研特惠价：¥498 | 现货库存（当日下午4点前订单当日发）

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注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Rapid Protein Quantification Kit (BCA Method) - KTD3010

Product Center: Cell Analysis | Category: Bestsellers | Catalog Number: KTD3010

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Core Applications

Specifically designed for protein samples, suitable for protein concentration determination in organelle extraction and staining experiments, compatible with biomedical research and in vitro analysis scenarios.

Kit Components

• Reagent A (Stable Chelator)
• Reagent B (Color Enhancer)
• BSA Standard (Pre-diluted Gradient)

Technical Advantages

• 5-minute Color Development: Achieves endpoint color reaction at room temperature, 4× faster than traditional BCA methods
• Ultra-wide Detection Range: 1-2000 µg/mL linear range, covering routine to trace sample detection
• Breakthrough Interference Resistance: Tolerates 5% SDS/Triton X-100/Tween series detergents
• Micro-sample Compatibility: Requires only 1-20 µL test sample, conserving precious specimens

Key Detection Parameters

Optimal Detection Wavelength	480 nm
Sensitivity Threshold	1 µg/mL
Color Stability	＞2 hours (room temperature, dark)

Operational Notes

1. Avoid reducing agent interference: Sample DTT/β-mercaptoethanol content must be ＜1 mM
2. Recommend simultaneous standard curve: Establish independent standard curves for each experiment
3. High-background sample treatment: Recommend switching to Bradford method kit (Catalog Number KTD3002)

▍ Storage & Shipping

• Storage Conditions: Components stored separately in dark, 12-month shelf life
• Shipping Protocol: Blue ice cold-chain transport ensures reagent stability

⚠ Important Notice

This product is for research use only, not for clinical diagnosis. Experimental operations must comply with local biosafety regulations. Validate protocol compliance before use.

Technical Background

Based on modified chelator technology, rapid color development is achieved through Cu<sup>+</sup>-specific complexation. In alkaline environments, protein molecules reduce Cu<sup>2+</sup> to Cu<sup>+</sup>, forming 480 nm characteristic absorption peak complexes with chelators, overcoming thermal stability limitations of traditional BCA methods.

Specifications & Delivery

500T Specifications | Research Special Price: ¥498 | In Stock (Orders before 4 PM shipped same day)

Note: Above information may change due to product optimization. Please consult Yakoyin Company for details.