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文献和实验Cloning of small RNAs with 5’ phosphate and 3’ OH ends
debris into a Spin-X Cellulose Acetate filter and spin at full speed for 2 minutes. Wash the gel bits once more with 100µl 0.3M NaCl and spin for another 2 min. Collect the 100µl eluate in the same tube. Add an equal volume (~600µl) of 100
. 5. Spin down the gel at 500xg for 5 min. Remove the supernatant and wash the gel with 3 M guanidine-HCl containing 0.5 M sodium acetate. Load it into the column. 6. Remove the guanidine solution and wash the gel well with 10mM tris pH 7.5, 2 mM
Procedure: Days 1 and 2: Follow the liquid or plate lysate protocol to prepare a lysate. Dilute the lysate by the appropriate amount as stated in the lysate procedure. Day 3 Spin the tubes at 2500 rpm for 30 minutes at room temperature
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