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- Biomatik
- RPU42385
- 2025年07月09日
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文献和实验DETECTION OF RECOMBINANT PROTEINS BY ELISA
of interest. If the recombinant protein is not soluble in H2 O, dissolve the insoluble pellet in a volume of 7 M urea equal to 1/10 of the bacterial culture volume. Vortex the samples at a medium speed for about 30 minutes (use a vortex setting of 4.5 on the Fisher Vortex Genie
Expression of Recombinant Proteinase Inhibitors in Plants
18 b Potato proteinase inhibitor II Ser 19 b Tomato proteinase inhibitor
Expression and Purification of Recombinant -Defensins and -Defensin Precursors in Escherichia coli
Recombinant expression of α-defensins can be obtained at efficient levels in Escherichia coli. Amplified α-defensin or pro-α-defensin coding cDNA sequences are cloned directionally between EcoRI and SalI sites of the pET-28a expression vector
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