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文献和实验Isolation of DNA from Agarose Gels(Paper Slurry Method)
Isolation of DNA from Agarose Gels (Paper Slurry Method) This procedure isolates DNA from agarose gels by filtration through a filter-paper column.The column is made in a 500 µL tube from a slurry of filter paper in TE buffer. Materials •Whatman
Fastfilter Plasmid Midi Kit Spin Protocol
2. Pellet up to 30-50 ml bacteria in appropriate vessels by centrifugation at 3,500-5,000 x g for 10 min at room temperature. 3. Decant or aspirate medium and discard. To ensure that all traces of the medium are removed, use a clean paper towel to blot
Fastfilter Plasmid Maxi Kit Spin Protocol
2. Pellet up to 100-200 ml bacteria in appropriate vessels by centrifugation at 3,500-5,000 × g for 10 min at room temperature. 3. Decant or aspirate medium and discard. To ensure that all traces of the medium are removed, use a clean paper towel
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