Pierce Color Silver Stain Kit

Protocol for Silver Staining

and you are not able to scale up your isolation of protein to reach coomasie stainable levels,you must use a mass spec compatible silver stain protocol.You must only use methanol and acetic acid during the fixing step.You must not use any solutions containing

SILVER STAINING OF NUCLEOLAR ORGANIZING REGIONS (NOR)

mat or paper towels since silver nitrate is a hazardous chemical that may cause burns, In addition, if it contacts the skin it will stain so un-noticed spills can cause problems for other workers. C. Using pasteur pipet, measure 3 drops of gelatin

Use of Immunogold with Silver Enhancement

will stain tissue red (1 , 2 ). However, unless the gold is silver-enhanced to help visualize it, the sensitivity of the staining is fairly low. During silver-enhancement, the colloidal gold serves as a nucleation site for the deposition of metallic silver