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文献和实验Silver Stain is about 10 to 100 times more sensitive than Coomasie Blue Staining; at least 1ng protein using this protocol. Estimation of protein amouts is not advisable since the staining intensity depends of the developing time and vary
Fix gel in 50% methanol for greater than 1 hour. rinse gel in dH2O 3 times 5 minutes each place back in 50% methanol for 1 hour. Stain gel in 100 mls of stain solution for 30 minutes Rinse gel in dH2O 3 times 5 minutes each. Place gel
SILVER STAIN OF PROTEIN GELS(Ⅱ)
1.Fix gel in 50% methanol for greater than 1 hour rinse gel in dH2O 3 times 5 minutes each place back in 50% methanol for 1 hour. 2.Stain gel in 100 mls of stain solution for 30 minutes 3.Rinse gel in dH2O 3 times 5 minutes each. 4.Place gel
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