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文献和实验Stewart Laboratory Standard Operating Procedure
alone stain is desired, set aside a fraction of cells until step 10). Resuspend cell pellet in anti-BrdU antibody (50 μL total solution diluted with wash buffer). Incubate for 20 minutes at 4°C. Use BrdU-FITC: Boeringher #1202693 1:100 dilution (skip to step
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
dipense in 40ul aliquots in pre-chilled, sterile eppis, freeze in lN2 and store -70C AGROBACTERIUM ELECTROPORATION Safety precautions 1. Never change any other settings than stated while unit is charging (this may damage
Stewart Laboratory Standard Operating Procedure
Stewart Laboratory Standard Operating Procedure BrdU Incorporation Protocol Day 1 Label cells with media containing100 μM BrdU (6 μL/mL of 5 mg/mL stock): Diploid Fibroblasts, 4-6 hours
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