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文献和实验Steps for Standard PCR Reaction Design primers. In general, primers should have the following properties: Length of 18-24 bases 40-60% G/C content Start and end with 1-2 G/C pairs
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
is off. 2. With Charge/Pulse switch of the electroporator in the PULSE position, connect the leads from the power supply to the corresponding coloured terminals on the back of the electroporator. 3. Set Arm/Disarm dial to disarmed 4. Set Capacitance
Procedures for In Vitro Production of Bovine Embryos
Procedures for In Vitro Production of Bovine EmbryosThe procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC)] of embryos described here are based on procedures developed
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