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文献和实验Isolation of Microtubules (Bovine Brain)
minutes at 4° C for further clarification. Collect 10 ml of the supernatant and determine the protein content of your sample using the Bradford protein assay (Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard
Pre-hyb/hyb for Radioactive Probes
Pre-hyb/hyb for Radioactive Probes Stuff you need: 50X Denhardt's reagent: 1% Ficoll 1% Polyvinylpyrrolidone 1% Bovine Serum Albumin Prehyb/hyb solution: 5X SSC 50% formamide 100 µg/ml SS DNA (heat denatured at 95 deg C) 5X
Stewart Laboratory Standard Operating Procedure
alone stain is desired, set aside a fraction of cells until step 10). Resuspend cell pellet in anti-BrdU antibody (50 μL total solution diluted with wash buffer). Incubate for 20 minutes at 4°C. Use BrdU-FITC: Boeringher #1202693 1:100 dilution (skip to step
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