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文献和实验Mag-Bind® mRNA Protocol (Standard Protocol)
实验试剂 1. 80% Ethanol 2. Isopropanol 3. Chloroform 实验设备 1. Magnetic Stand for 1.5 ml tube (OBI # MSD-02) 2. Nuclease-free 1.5 and 2 ml centrifuge tubes 3. Microcentrifuge
Kingfisher Flex 96 Plant DNA High Pure Protocol
for pipette 7. Ice Bucket 8. 1.5 or 2.0 mL microcentrifuge tubes, sealed deep-well plate or capped microtube rack for sample disruption 9. Kingfisher 96 or Kingfisher Flex 96 with Deep Well Magnet 10. 4 Deep Well Kingfisher 96 Plates
Dynabeads® mRNA DIRECT Micro Kit - mRNA isolation for RT-PCR amplification
Dynabeads® Oligo (dT)25 Lysis/Binding Buffer Washing Buffer A Washing Buffer B 实验设备 Dynal® magnet: see www.invitrogen.com/magnets Sterile, RNase-free micro centrifuge tubes Sterile, RNase-free pipette
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