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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
Microcentrifuge tubes Micropipettor and tips Rotator Sonicator Spectrometer (Qubit Systems) Test tubes, conical, 50- and 250-mL Thermal cycler Tubes (microcentrifuge) Vortex mixer Water baths or heating
into 12 X 75 mm Falcon tubes containing 1.5 ml aliquots of the cells. Incubate for 4-6 hours at 37degC with shaking at 250 rpm. 2. Transfer the culture to 1.5 ml microcentrifuge tubes and centrifuge for 15 minutes at 12,000 rpm at 4degC.
Kingfisher Flex 96 Plant DNA High Pure Protocol
for pipette 7. Ice Bucket 8. 1.5 or 2.0 mL microcentrifuge tubes, sealed deep-well plate or capped microtube rack for sample disruption 9. Kingfisher 96 or Kingfisher Flex 96 with Deep Well Magnet 10. 4 Deep Well Kingfisher 96 Plates
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