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文献和实验DNA is dissolved in Hepes buffer (10 mM, pH 7.0) at a final concentration of 20 m g/ml. Cationic polymers are dissolved in pure water (2.5 mg/ml). An appropriate volume of cationic polymer solution is added at room
Coating DNA with RecA Protein to Distinguish DNA Path by Electron Microscopy
the overpassing and underpassing segments when two DNA helices cross. Conventional microscopy rarely allows such resolution. Coating DNA with RecA protein (1 ,2 ) allows the unambiguous determination of a DNA crossover (3 -5 ).
Silane and Poly-L-Lysine Coating of Microscope Slides
free environment for 5 minutes. Poly-L-Lysine Coating a) Smear 20ul of poly-l-lysine over each slide. b) Air dry in a dust free environment. Silated Slides a) To 300mls acetone add 6mls Aminopropyltriethoxysilane solution (Sigma
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