Active Ras Pull-Down and Detec

Pull-Down Assays for Guanosine 5-Triphosphate-Bound Ras-Like Guanosine 5-Triphosphatases

Small guanosine 5′-triphosphatases (GTPases) of the Ras superfamily switch between a guanosine 5′-diphosphate (GDP)-bound and a guanosine 5′-triphosphate (GTP)-bound conformation. This cycle is regulated by guanine nucleotide exchange factors

Detection of ras Gene Mutations Using Oligonucleotide Ligation Technology

mutations is to lock the protein in a GTP-bound, active conformation, thus perturbing cellular physiology and contributing to tumorigenesis. Different tumor types show specificity of ras oncogene activation. For example, activated H-ras occurs

DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE

) upon reaction with free ferric ion. Do not use metal forceps to manipulate the tissue while it is in the X-gal detection buffer. iv. All tissues have endogenous, lysozomal ß-gal. Its pH optimum is very low, and thus it is not very active in the pH