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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
) upon reaction with free ferric ion. Do not use metal forceps to manipulate the tissue while it is in the X-gal detection buffer. iv. All tissues have endogenous, lysozomal ß-gal. Its pH optimum is very low, and thus it is not very active in the pH
for the siRNA targeted gene compared to the respective control(s).Part III: Detection of pRNAs by 5'biotin EF52 pull-down assay 1.A minimum of ~4.0×10 6 cells are required/sample. Twenty-four hours following the plating (~70% confluent) the cultures
Rac and Rap GTPase Activation Assays
- and GTP-bound states and highly specific antibodies are not yet available for many small GTPases, analysis of certain members of the Rho and Ras GTPase family are now routinely performed. Here, we describe affinity-based pull-down assays for detection
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