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文献和实验v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
a convenient source of these proteins for biological studies. Yields of protein vary; screening of clonallines for high expression is usually worthwhile. Heparin affinity chromatography is an easy step thatprovides a major enrichment, particularly for sFRP
Studies of the Ubiquitin Proteasome System
protocol 4214 and protocol 4315 ) Glutathione‐Sepharose 4B beads (50% slurry; GE Healthcare) Phosphate‐buffered saline (PBS; see recipe ), 4°C Binding buffer
Measurement of Oxygen Radicals and Lipid Peroxidation in Neural Tissues
Basic Protocol 4: HPLC Assay of Glutathione Basic Protocol 5: HPLC‐Chemiluminescence Assay of Lipid Hydroperoxides Support Protocol 1: Xylenol Orange Determination of Hydroperoxide Content in Standards
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