Pierce Glutathione Chromatogra

GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins

chromatography column Glutathione-Sepharose beads Sonicator Additional Reagents This protocol also requires equipment and reagents for SDS-PAGE, including Coomassie blue (see Sambrook and Russell 2001) SDS-PAGE sample buffer, 2x (Sambrook and Russell

Determination of Organophosphorus Pesticide Residues in Vegetable Oils by Single-Step Multicartridge Extraction and Cleanup and by Gas Chromatography

of a system of three cartridges. A solution of 1 g oil in n -hexane is loaded into an Extrelut-NT3 cartridge (large-pore diatomaceous material). The OP pesticide residues are extracted by eluting the cartridge with 20 mL acetonitrile, which is cleaned up by passing

GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins

Centrifuge, precooled to 4°C Shaking incubator, preset to 37°C Rotator for end-over-end mixing Disposable chromatography column Glutathione-Sepharose beads Sonicator