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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
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- 保质期:
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- 英文名:
Lactase
- 库存:
咨询
- 供应商:
无锡云萃生物
- CAS号:
9031-11-2
- 规格:
2mg/10mg/500mg/500g
| 规格: | 2mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
| 规格: | 500g | 产品价格: | ¥100.0 |
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文献和实验HIS-tagged protein purification
and wash resin 3x in 50ml UPB6.3 w/5' gentle shaking each time. 7)resuspend in 25ml UPB6.3,pour into column and elute to top with another 25ml.s 8)elute bound proteins 20ml w/ UPB6.3+250mM imidazole. 9)lN2 freeze and store at 70℃ UPB 1 liter [final
chloride,citrate,cysteine,diethanolamine,dithiothreitol,EDTA,EGTA,HEPES,mercaptoethanol,Nonidet P-40,phenol,polyvinyl pyrrolidone,sodium deoxycholate,sodium salicylate,thimerosol,Tricine,TRIS and Triton X-100.There is also much protein-to-protein variation
Protein Expression and Purification Protocol
to appear. Step 2: Make a starter culture for protein expression. Usually to about 250 to 500 mL of LB broth the antibiotic is added. a) Generally, the antibiotic is ampicillin, which is added to the LB broth at 100 m g/ml final concentration










