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2252168-06-0
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Pyrene azide 3
CAS No. : 2252168-06-0
MCE 国际站：Pyrene azide 3
产品活性：Pyrene azide 3 是一种 PROTAC linker，属于 PEG 类。可用于合成 PROTAC 分子。Pyrene azide 3 是一种点击化学试剂。它含有 Azide 基团，可以和含有 Alkyne 基团的分子发生铜催化的叠氮-炔环加成反应（CuAAc)。还可以和含有 DBCO 或 BCN 基团的分子发生菌株促进的炔-叠氮环加成反应 (SPAAC)。
研究领域：PROTAC
作用靶点：PROTAC Linkers
In Vitro: PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.
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文献和实验Purification of Antiserum or Ascites by Protein A/G Chromatography
PreparationTo prepare 1 liter of TBS (50 mM Tris-HCl, pH 7.4; 150 mM NaCl; 0.05% sodium azide) add the following to 800ml of distilled water:Ⅰ6.06 g of Tris baseⅡ8.77 g of NaClⅢ 10ml of 5% sodium azide stock solution (Do not add if the antibody will used
常用化学试剂分子量 Molecular Weights of Commonly Used Chemicals
acid) 131.18 Magnesium Sulfate, anhydrous 120.39 Ammonium Acetate 77.08 Pepstatin-A 685.9 Ammonium Bicarbonate 79.06 Phenol
IMMUNOSCREENING OF WITH ANTIBODY PROBES
dry milk solution made in TBST (or block overnight at 4°C). Wash membranes four times in TBST for 5 minutes each at room temperature. Dilute serum 1:100 in 3% BSA and 0.02% sodium azide in TBST Transfer diluted serum in a 150 mm Petri dish. Place
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