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- QIAGEN
- 47016
- 2026年01月27日
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- 技术资料
- 库存：
9999
- 保质期：
见包装
- 供应商：
武汉科昊佳生物科技有限公司
- 保存条件：
常温
| 品名 | 货号 | 品牌 | 规格 |
| Qiagen 47014 强力土壤基因组DNA提取试剂盒（50） | 47014 | Qiagen | 50T |
| Qiagen 47016强力土壤基因组DNA提取试剂盒（250） | 47016 | Qiagen | 250T |
Introduction
The DNeasy PowerSoil Pro Kit comprises a novel and proprietary method for isolating microbial genomic DNA from environmental samples. The kit uses QIAGEN’s second- generation Inhibitor Removal Technology® (IRT) and is intended for use with environmental samples containing high humic acid content, including difficult soil types such as compost, sediment, and manure. Other more common soil and stool types have also been used successfully with this kit. Improved IRT combined with more efficient bead beating and lysis chemistry yields high-quality DNA that can be used immediately in downstream applications, including PCR, qPCR, and next-generation sequencing (16S and whole genome).
| DNeasy PowerSoil Pro Kit Catalog no. Number of preps |
(50) 47014 50 |
(250) 47016 250 |
| PowerBead Pro Tubes | 50 | 250 |
| MB Spin Columns | 50 | 250 |
| Solution CD1 | 40 ml | 200 ml |
| Solution CD2 | 15 ml | 60 ml |
| Solution CD3 | 35 ml | 175 ml |
| Solution EA | 36 ml | 175 ml |
| Solution C5 | 30 ml | 175 ml |
| Solution C6 | 9 ml | 66 ml |
| Microcentrifuge Tubes (2 ml) | 100 | 500 |
| Elution Tubes (1.5 ml) | 50 | 250 |
| Collection Tubes (2 ml) | 100 | 500 |
| Quick-Start Protocol | 1 | 1 |
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文献和实验in the Beckman. Promptly pour supernatant into another round-bottom 13 ml polycarbonate tube and re-centrifuge at 20,000 x g for 15 min. While the centrifugation is happening, equilibrate a Qiagen-tip 100 (see next step). 7. To equilibrate the tip
Buffer P1 50 mM Tris-HCl pH 8.0 10 mM EDTA 100 μg/ml RNaseA The buffer and RNaseA can also be ordered from Qiagen separately (catalog numbers 19051 and 19101). Buffer P2 200 mM NaOH 1% SDS Buffer P3
Qiagen MIDI protocol(QIAGEN plasmid Midi试剂盒)
and re-centrifuge at 20,000 x g for 15 min. While the centrifugation is happening, equilibrate a Qiagen-tip 100 (see next step). 7. To equilibrate the tip, put it in a plastic collar and put this assembly into the top of a 50-ml conical polypropylene tube. Add
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