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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
12个月
- 英文名:
Recombinant Streptococcus pyogenes serotype M28 Holo-[acyl-carrier-protein] synthase (acpS)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10ug/20ug/50ug/100ug/200ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥69.0 |
|---|---|---|---|
| 规格: | 20ug | 产品价格: | ¥139.0 |
| 规格: | 50ug | 产品价格: | ¥209.0 |
| 规格: | 100ug | 产品价格: | ¥349.0 |
| 规格: | 200ug | 产品价格: | ¥419.0 |
| 规格: | 500ug | 产品价格: | ¥559.0 |
| 规格: | 1mg | 产品价格: | ¥699.0 |
Research Areas
OthersUniprot ID
Q48RM7Gene Names
acpSAlternative Name(s)
4'-phosphopantetheinyl transferase AcpS (Holo-ACP synthase)Lead Time
3-7 business daysOrganism
Streptococcus pyogenes serotype M28 (strain MGAS6180)Source
BaculovirusExpression Region
1-118aaProtein Length
Full LengthTag Info
N-terminal 10xHis-tagged and C-terminal Myc-taggedTarget Protein Sequence
MIVGHGIDLQEISAIEKVYQRNPRFAQKILTEQELAIFESFPYKRRLSYLAGRWAGKEAFAKAIGTGIGRLTFQDIEILNDVRGCPILTKSPFKGNSFISISHSGNYVQASVILEDKKMW
17.1 kDaPurity
Greater than 90% as determined by SDS-PAGE.Form
Liquid or Lyophilized powderBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验of fatty acid synthesis inhibitors (refs. 7,8). Materials Reagents DTT was from Fisher (BP172-5); beta-mercaptoethanol was from Bio-Rad (161-0710). [2-14C]Malonyl-CoA (60 mCi/mmol, Perkin Elmer, NEC612), ACP (Sigma, A7303) was pretreated with 3 mM DTT
Gel-elongation assay for type II fatty acid synthesis
(>6C) acyl-ACPs. With addition of cerulenin (200 µg/ml) or platensimycin (167 µg/ml), the reaction was blocked showing major accumulation of butyryl-ACP (4:0-ACP). With thiolactomycin (333 µg/ml), more malonyl-ACP and less butyryl-ACP were accumulated
Gel-elongation assay for type II fatty acid s
the reaction was completed and produced final products of long chain (>6C) acyl-ACPs. With addition of cerulenin (200 µg/ml) or platensimycin (167 µg/ml), the reaction was blocked showing major accumulation of butyryl-ACP (4:0-ACP). With thiolactomycin (333 µg
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