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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Leiurus hebraeus Alpha-like toxin Lqh6
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Leiurus hebraeus Alpha-like toxin Lqh6品名简称:
Recombinant Leiurus hebraeus Alpha-like toxin Lqh6 protein货号:
CSB-BP348580LDS规格:
20μg价格:
3168LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
N/AUniprot No.:
P59356别名:
Lqh VI (LqhVI)种属:
Leiurus hebraeus (Deathstalker scorpion) (Leiurus quinquestriatus hebraeus)蛋白长度:
Full Length来源:
Baculovirus分子量:
10.7 kDa表达区域:
1-64aa氨基酸序列:
VRDGYIAQPENCVYHCIPDCDTLCKDNGGTGGHCGFKLGHGIACWCNALPDNVGIIVDGVKCHK蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
28-38 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Alpha toxins bind voltage-independently at site-3 of sodium channels and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. This toxin is highly toxic to insects and mice, and inhibits the binding of alpha-toxin to cockroach neuronal membranes.功能2:
/文献:
"Characterization of scorpion alpha-like toxin group using two new toxins from the scorpion Leiurus quinquestriatus hebraeus." Hamon A., Gilles N., Sautiere P., Martinage A., Kopeyan C., Ulens C., Tytgat J., Lancelin J.-M., Gordon D. Eur. J. Biochem. 269:3920-3933(2002)研究领域:
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文献和实验下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。
层析复性蛋白质 李明1 苏志国1 Janson Jan-Christer2 (1 生化工程国家重点实验室,中国科学院过程工程研究所,北京,100080 2 表面生物技术系,Uppsala 大学,Uppsala, 瑞典) 摘要在生物学和生物医学中,尤其在制药行业中,蛋白质的体外折叠复性是一个比较棘手的问题。最近利用层析方法复性成为研究的热点,本综述主要介绍层析复性方法的进展。 随着基因工程和生物技术的发展,蛋白质克隆与表达已经趋于成熟。在重组蛋白质生产的过程中,目标产物的异源
坏死因子(Tumornecrosisfactor,TNF)根据其产生来源和结构不同,可分为TNF-α和TNF-β两类,前者由单核-巨噬细胞产生,后者由活化T细胞产生,又名淋巴毒素。TNF除具有杀伤肿瘤细胞外,还有免疫调节、参与发热和炎症的发生。 5,转化生长因子-β家族(Transforminggrowthfactor-βfamily,TGF-βfamily)主要包括TGF-β1、TGF-β2、TGF-β3、TGFβ1β2以及骨形成蛋白(BMP)等。 6.趋化因子家族(Chemokinefamily)包括两个亚族:(1)C-X-C
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