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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Moraxella bovis Fimbrial protein Q (tfpQ)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Moraxella bovis Fimbrial protein Q (tfpQ)品名简称:
Recombinant Moraxella bovis tfpQ protein货号:
CSB-EP357167MHZ规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
tfpQUniprot No.:
P07640别名:
Beta pilin (Q pilin)种属:
Moraxella bovis蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
20.9 kDa表达区域:
7-157aa氨基酸序列:
FTLIELMIVIAIIGILAAIALPAYQDYISKSQTTRVVGELAAGKTAVDAALFEGKTPKLGKAANDTEEDIGLTTTGGTARSNLMSSVNIGGGAFATGAGTLEATLGNRANKDIAGAVITQSRDAEGVWTCTINGSAAPGWKSKFVPTGCKE蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Moraxella bovis can express either a Q or a I pilin, the inversion of 2 kb of DNA determines which pilin is expressed.功能2:
/文献:
"Purification, characterization, and pathogenicity of Moraxella bovis pili." Ruehl W.W., Marrs C.F., Fernandez R., Falkow S., Schoolnik G.K. J. Exp. Med. 168:983-1002(1988)研究领域:
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文献和实验本帖姐妹篇: 【原创】蛋白质纯化武器-设备篇(欢迎补充) http://www.dxy.cn/bbs/post/view?bid=65&id=15120322&sty=3&keywords=%B4%BF%BB%AF%CE%E4%C6%F7 (这篇小文可是上过丁香园的头版哦,还曾给配了个好看的图片,感谢) 【资源】蛋白质纯化武器-资料篇(欢迎补充) http://protein.dxy.cn/bbs/topic/17703087 条条大路通罗马,纯化之路千万条。本篇是写给蛋白
条条大路通罗马,纯化之路千万条。本篇是写给蛋白质纯化新手的,就一些最常用的纯化工艺、概念方法作一些列举。以起始原料的不同来进行分类,分为:大肠杆菌、酵母、细胞培养、腹水、血清、生物组织六个板块。 板块一、大肠杆菌 (这里讨论的大肠杆菌为非分泌到培养基中的重组蛋白,是否有重组蛋白分泌到培养基中的工程菌我没有见过。) 一、关于菌体的量 大肠杆菌表达的基因工程蛋白是纯化人员最方便获得的原料,对纯化工艺开发来说几乎没有原料方面的限制。常看到有战友用个几毫升的菌液去做纯化,对此
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