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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
12个月
- 英文名:
Recombinant Escherichia coli Universal stress protein D (uspD)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10ug/20ug/50ug/100ug/200ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥69.0 |
|---|---|---|---|
| 规格: | 20ug | 产品价格: | ¥139.0 |
| 规格: | 50ug | 产品价格: | ¥209.0 |
| 规格: | 100ug | 产品价格: | ¥349.0 |
| 规格: | 200ug | 产品价格: | ¥419.0 |
| 规格: | 500ug | 产品价格: | ¥559.0 |
| 规格: | 1mg | 产品价格: | ¥699.0 |
Research Areas
OthersUniprot ID
P0AAB8Gene Names
uspDAlternative Name(s)
yiiTLead Time
3-7 business daysOrganism
Escherichia coli (strain K12)Source
E.coliExpression Region
1-142aaProtein Length
Full LengthTag Info
N-terminal 6xHis-taggedTarget Protein Sequence
MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLSELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKEQCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDKMW
20.4 kDaPurity
Greater than 85% as determined by SDS-PAGE.Form
Liquid or Lyophilized powderBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验We describe methods for screening the E. coli ASKA overexpression library for clones that lead to altered expression of reporter genes. First, a promoter of interest is cloned upstream of either the lacZ or luxCDABE genes to yield reporter
Renaturation of the Catalytic Domain of PDE4A Expressed in Escherichia coli as Inclusion Bodies
Owing to simplicity, speed, cost advantage, and a generally high product yield, expression in Escherichia coli is the method of choice for the production of large amounts of protein. However, because of the high expression level, proteins
The Universal Primers and the Shotgun DNA Sequencing Method
For studies in molecular biology, DNA purification has been essential, in particular for DNA sequencing, probing, and mutagenesis. The amplification of DNA in Escherichia coli by cloning vehicles derived from M13mp or pUC made expensive
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