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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Arabidopsis thaliana Dehydrin ERD14 (ERD14)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14796.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4374.0 |
| 规格: | 20μg | 产品价格: | ¥2328.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
ERD14Uniprot No.:
P42763别名:
ERD14; At1g76180; T23E18.12; T23E18_36Dehydrin ERD14种属:
Arabidopsis thaliana (Mouse-ear cress)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
28.1 kDa表达区域:
2-185aa氨基酸序列:
AEEIKNVPEQEVPKVATEESSAEVTDRGLFDFLGKKKDETKPEETPIASEFEQKVHISEPEPEVKHESLLEKLHRSDSSSSSSSEEEGSDGEKRKKKKEKKKPTTEVEVKEEEKKGFMEKLKEKLPGHKKPEDGSAVAAAPVVVPPPVEEAHPVEKKGILEKIKEKLPGYHPKTTVEEEKKDKE蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Intrinsically disordered protein acting as a chaperone. Prevents heat-induced aggregation and/or inactivation of various substrates. Binds to acidic phospholipid vesicles without affecting membrane fluidity.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
In Stock ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003)Function:
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文献和实验Introduction of human 14-3-3 proteins
of their association with the G-box DNA/protein complex by a monoclonal antibody screening approach.GF14 proteins are homologous to the 14-3-3 family of mammalian proteins.Here we demonstrate that recombinant GF14 omega,one member of the Arabidopsis GF14 protein family
Protocol for vacuum-infiltration transformation of Arabidopsis thaliana
papers : Bouchez D, Camilleri C, Caboche M (1993). A binary vector based on Basta resistance for in planta transformation of Arabidopsis thaliana. C R Acad Sci Paris, Sciences de la vie/Life sciences, 316 : (in press, October issue). Bechtold N
Chromatin Immunoprecipitation Protocol to Analyze Histone Modifications in Arabidopsis thaliana
modifications in the plant model organism Arabidopsis thaliana. This protocol is an adapted version of the original procedure published by Lawrence and co-workers (Lawrence et al. , 2004). Back to top Procedure Plant Material Arabidopsis
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