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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
12个月
- 英文名:
Recombinant Nostoc sp. Protein patA (patA)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10ug/20ug/50ug/100ug/200ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥69.0 |
|---|---|---|---|
| 规格: | 20ug | 产品价格: | ¥139.0 |
| 规格: | 50ug | 产品价格: | ¥209.0 |
| 规格: | 100ug | 产品价格: | ¥349.0 |
| 规格: | 200ug | 产品价格: | ¥419.0 |
| 规格: | 500ug | 产品价格: | ¥559.0 |
| 规格: | 1mg | 产品价格: | ¥699.0 |
Research Areas
OthersUniprot ID
P39048Gene Names
patAAlternative Name(s)
PAT-1Lead Time
3-7 business daysOrganism
Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)Source
E.coliExpression Region
1-379aaProtein Length
Full LengthTag Info
N-terminal 10xHis-tagged and C-terminal Myc-taggedTarget Protein Sequence
MKTLPITRYRFFQKIQPLSLLKKITGKTITGCLQVFSTSGTWSIYVEEGKLIYACYSERMFEPLYRHLGNLSPQIATLPKEINEQLRAIFETGIENQAIPNPDYLAICWLVNQKYISSSQAAVLIEQLALEVVESFLMLEEGSYEFIPESFLDDLPKFCYLNVRLLVEQCQQHGRVPEAFRREASSQEISSSTEHNQIPVNNRRSTKFTSPPHTQPKPEPRLPQINTNKSTEYSKRYASQPNTVNHGYSQTSATSTDKKIYTIFCIDENPIVLNNIKNFLDDQIFAVIGVTDSLKALMEILCTKPDIILINVDMPDLDGYELCSLLRKHSYFKNTPVIMVTEKAGLVDRARAKIVRASGHLTKPFNQGDLLKVIFKHITMW
50.2 kDaPurity
Greater than 85% as determined by SDS-PAGE.Form
Lyophilized powder.If the size you ordered does not have lyophilized powder in stock, we will provide it in liquid formBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验【信息】Northstar BioProducts(NSBP)
, (Pseudomonas sp.), EC 3.2.1.103 100812-1 Keratanase II, (Bacillus sp.) 100686-1 O-Glycosidase, (Streptococcus pneumonia recombinant), EC 3.2.1.97 100688-1 PNGase F, (Chryseobacterium menigosepticum), EC 3.5.1.52 120389-1 -Glucosaminidase
方案。纯化的方法我们有层析、沉淀、萃取、透析、超滤等等。 柱层析是纯化最强有力的手段,几乎每种蛋白的纯化都会用到层析。各种层析方法:如亲和、离子交换、疏水、反相、分子筛等等都是我们工艺宝库里的吃饭家伙。不知道怎么吃啊,没办法了,你还是先去补一补基础知识吧。GE公司的一套书个人认为是最佳的入门资料,如Recombinant Protein Purification,Affinity Chromatography,Ion-Exchange chromatography,Gel Filtration
,符合你纯化的要求就是硬道理。纯化方法不好讲,各种蛋白不一样,纯化工艺自然不同;即使相同的原料也有不同的纯化方案。纯化的方法我们有层析、沉淀、萃取、透析、超滤等等。 柱层析是纯化最强有力的手段,几乎每种蛋白的纯化都会用到层析。各种层析方法:如亲和、离子交换、疏水、反相、分子筛等等都是我们工艺宝库里的吃饭家伙。不知道怎么吃啊,没办法了,你还是先去补一补基础知识吧。GE公司的一套书个人认为是最佳的入门资料,如Recombinant Protein Purification,Affinity
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