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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Major outer membrane lipoprotein Lpp (lpp)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Escherichia coli Major outer membrane lipoprotein Lpp (lpp)品名简称:
Recombinant E.coli lpp protein货号:
CSB-EP300885ENV规格:
20μg价格:
2328LC-MS/Western Blot:
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP300885ENV could indicate that this peptide derived from E.coli-expressedSDS-PAGE:
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP300885ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) lpp.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
lppUniprot No.:
P69776别名:
Braun lipoprotein (Murein-lipoprotein) (mlpA) (mulI)种属:
Escherichia coli (strain K12)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
21.8 kDa表达区域:
21-78aa氨基酸序列:
CSSNAKIDQLSSDVQTLNAKVDQLSNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK蛋白标签:
N-terminal 6xHis-KSI-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Interacts with the peptidoglycan both covalently and noncovalently. This interaction contributes to the maintenance of the structural and functional integrity of the cell envelope.功能2:
Interacts with the peptidoglycan both covalently and noncovalently. This interaction contributes to the maintenance of the structural and functional integrity of the cell envelope.文献:
"Roles of the hydrophobic cavity and lid of LolA in the lipoprotein transfer reaction in Escherichia coli." Watanabe S., Matsuyama S., Tokuda H. J. Biol. Chem. 281:3335-3342(2006)研究领域:
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文献和实验相关专题 大肠杆菌的基因工程 基因工程让一切皆有可能 一、原理 1、 E . coli 表达系统 E . coli 是重要的原核表达体系。在重组 基因转化入E . coli 菌株以后,通过温度的控制,诱导其在宿主菌内表达目的蛋白质,将表达样品进行SDS-PAGE 以检测表达蛋白质。 2、 外源基因的诱导表达 提高外源基因表达 水平的基本手段之一,就是将宿主菌的生长与外源基因的表达分成
概要 对于基因工程技术构建的重组蛋白,通常分为可溶性重组蛋白和不溶性重组蛋白,可溶性的重组蛋白一般常常存在于细胞质内、细胞分泌到培养基、分泌到周质间隙中等。不可溶性的蛋白主要是由于表达系统的高效表达,在细胞内形成了包涵体。E.coli是应用最为广泛的重组蛋白质表达系统,但是表达的外源性蛋白多以包涵体的形式存在,为了重新获得生物活性,需要对包涵体蛋白质进行体外变复性操作。 蛋白质复性模型 根据对蛋白质折叠过程的研究,目前科学家已提出多种蛋白质复性模型,折叠漏斗模型是目前被广泛认可
上,而质粒是一种可转移的环状 DNA,很容易在不同菌株之间传递和交换遗传物质。 人随身携带十倍于细胞数的微生物,有益菌也有有害菌; 如果调皮的质粒带着基因在这些微生物中乱窜; 那我们的健康真是时时刻刻受到威胁!!! 对于身体健康而言真可谓 美国微生物学会刊物《抗生素与化疗》中一篇文章指出,E.coli MRSN 388634 的质粒中编码了包括 MCR-1 在内的 15 种耐药基因,但 E.coli MRSN 388634 并非对所有抗
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