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Alternative name(s):
Complex I-B12; CI-B12
NADH-ubiquinone oxidoreductase B12 subunit](https://img1.dxycdn.com/p/s14/2024/1230/657/0069886745579563781.jpg!wh200)
Recombinant-Pan-troglodytes-NADH-dehydrogenase-ubiquinone-1-beta-subcomplex-subunit-3NDUFB3NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 Alternative name(s): Complex I-B12; CI-B12 NADH-ubiquinone oxidoreductase B12 subunit
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Major outer membrane lipoprotein Lpp (lpp)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14796.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4374.0 |
| 规格: | 20μg | 产品价格: | ¥2328.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
lppUniprot No.:
P69776别名:
Braun lipoprotein (Murein-lipoprotein) (mlpA) (mulI)种属:
Escherichia coli (strain K12)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
21.8 kDa表达区域:
21-78aa氨基酸序列:
CSSNAKIDQLSSDVQTLNAKVDQLSNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK蛋白标签:
N-terminal 6xHis-KSI-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Interacts with the peptidoglycan both covalently and noncovalently. This interaction contributes to the maintenance of the structural and functional integrity of the cell envelope.内毒素:
Not test.SDS-PAGE:
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP300885ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) lpp.LC-MS Image Description/Western Blot:
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP300885ENV could indicate that this peptide derived from E.coli-expressedProduct types:
In Stock ProteinBiological_Activity:
/Research Areas:
Signal TransductionReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Roles of the hydrophobic cavity and lid of LolA in the lipoprotein transfer reaction in Escherichia coli." Watanabe S., Matsuyama S., Tokuda H. J. Biol. Chem. 281:3335-3342(2006)Function:
Interacts with the peptidoglycan both covalently and noncovalently. This interaction contributes to the maintenance of the structural and functional integrity of the cell envelope.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Isolation of Full-Length IgG Antibodies from Combinatorial Libraries Expressed in Escherichia coli
We have developed a technology for the facile isolation of full-length IgG antibodies with desired specificity from combinatorial libraries expressed in Escherichia coli . Full-length heavy and light chains are expressed from a bicistronic
and FimD usher proteins from the bacterial outer membrane, and the purification of PapD-PapG and FimC-FimH chaperone-�subunit complexes from the periplasm. These Pap and Fim proteins belong to the P and type 1 pilus systems of uropathogenic Escherichia coli
Strategies to Optimize Protein Expression in E. coli
. Protein Expr. Purif 58:210‐221. Grodberg, J. and Dunn, J.J. 1988. Ompt encodes the Escherichia coli outer‐membrane protease that cleaves T7‐Rna polymerase during purification. J
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