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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Zea mays Chitinase (chiA)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Zea mays Chitinase (chiA)品名简称:
Recombinant Zea mays chiA protein货号:
CSB-EP2939ZAX规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
chiAUniprot No.:
D0EM57别名:
/种属:
Zea mays (Maize)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
30.9 kDa表达区域:
27-278aa氨基酸序列:
QNCGCQPNFCCSKFGYCGTTDDYCGDGCQSGPCRSGGGGGGGGGGGSGGANVANVVSDAFFNGIKNQAGSGCEGKNFYTRSAFLSAVNKYPGFAHGGTEVEGKREIAAFFAHVTHETGHFCYISEINKSNAYCDASNRQWPCAAGQKYYGRGPLQISWNYNYGPAGRDIGFNGLADPNRVAQDAVIAFKTALWFWMNNVHRLMPQGFGATIRAINGALECNGNNPAQMNARVGYYKQYCQQLRVDPGPNLTC蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
"Allozyme-specific modification of a maize seed chitinase by a protein secreted by the fungal pathogen Stenocarpella maydis." Naumann T.A., Wicklow D.T. Phytopathology 100:645-654(2010)研究领域:
Immunology请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验D. E ., Pratt, R. C. and Finer, J. J. (1996) Intron-mediated enhancementof gene expression in maize (Zea mays L) and bluegrass (Poa pratensis L ). Plant Cell Rep. 15, 489-4 9 4 . 5. Rose, A. B. and Beliakoff, J.
条件下较高的本底表达,为了让表达系统严谨调控产物表达,能过量表达lacI阻遏蛋白的lacIq 突变菌株常被选为Lac/Tac/trc表达系统的表达菌株。现在的Lac/Tac/trc载体上通常还带有lacIq 基因,以表达更多lacI阻遏蛋白实现严谨的诱导调控。IPTG广泛用于诱导表达系统,但是IPTG有一定毒性,有人认为在制备医疗目的的重组蛋白并不合适,因而也有用乳糖代替IPTG作为诱导物的研究。另外一种研究方向是用lacI的温度敏感突变体,30度下抑制转录,42度开发。热诱导不用添加外来的诱导物,成本
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