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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Saccharomyces cerevisiae 3-methyl-2-oxobutanoate hydroxymethyltransferase (ECM31)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥17754.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4956.0 |
| 规格: | 20μg | 产品价格: | ¥2616.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
ECM31Uniprot No.:
P38122别名:
Extracellular matrix protein 31 (Ketopantoate hydroxymethyltransferase)种属:
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)蛋白长度:
Full Length来源:
Yeast分子量:
38.3 kDa表达区域:
1-312aa氨基酸序列:
MNIMKRQLCTSSKRFFSTAKNVVKYNTIQDIRNKYFTGTPLSMCTAYDFITATWVNKANCDLLLVGDSLAMTSLGYDSTITLSLNEFKYHVASVCRAEG_S_SMVVVDMPFGTFESGISDGLKNAIDIMKLDSKVTSVKVEVGSYTKDKYAMKFIEELCSRGIPVMAHIGLTPQKVHSLGGYKVQGSKSLLQMQELYETAMQLQKIGCWSILIECVPHKMAQFITSKLSVPTIGIGAGNGTSGQVLVISDLLGMQGDSVPKFVKQAVNMTDIATQGLKEYIASVEDRTFPERGTHTFKVKEDLWNEFLSSINEK蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
25-35 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
/内毒素:
Not test.SDS-PAGE:
Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP330827SVG could indicate that this peptide derived from Yeast-expressed Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ECM31.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
CancerReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae." Uetz P., Giot L., Cagney G., Mansfield T.A., Judson R.S., Knight J.R., Lockshon D., Narayan V., Srinivasan M., Pochart P., Qureshi-Emili A., Li Y., Godwin B., Conover D., Kalbfleisch T., Vijayadamodar G., Yang M., Johnston M., Fields S., Rothberg J.M. Nature 403:623-627(2000)Function:
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文献和实验Chromatin immunoprecipitation protocol for Saccharomyces cerevisiae
double-strand breaks (DSBs) in budding yeast (Saccharomyces cerevisiae ) (Cobb et al., 2003; van Attikum et al., 2004). Back to top Procedure Preculture of yeast cells for ChIP of proteins at DSBs We have used strains with JKM179
Studying DNATopoisomerase I-Targeted Drugs In the Yeast: Saccharomyces cerevisiae
The budding yeast Saccharomyces cerevisiae has been a valuable model in establishing eukaryotic DNA topoisomerase I as the cellular target of specific antineoplastic agents, including camptothecin (1 –3 ), aclacinomycin A (4 ), and R
Polyamine content in cells is regulated by biosynthesis, degradation, and transport. With regard to transport, uptake and excretion proteins exist in Escherichia coli and Saccharomyces cerevisiae . In E. coli , the uptake systems comprise
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