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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Heat-stable enterotoxin A2 (sta2)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14796.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4374.0 |
| 规格: | 20μg | 产品价格: | ¥2328.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
sta2Uniprot No.:
Q47185别名:
STA2种属:
Escherichia coli蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
18.1 kDa表达区域:
54-72aa氨基酸序列:
NSSNYCCELCCNPACTGCY蛋白标签:
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Toxin which activates the particulate form of guanylate cyclase and increases cyclic GMP levels within the host intestinal epithelial cells.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
In Stock ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Rectification of two Escherichia coli heat-stable enterotoxin allele sequences and lack of biological effect of changing the carboxy-terminal tyrosine to histidine." Guzman-Verduzio L.M., Kupersztoch Y.M. Infect. Immun. 57:645-648(1989)Function:
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文献和实验Mutant Heat-Labile Entertoxins As Adjuvants for CTL Induction
Heat-labile enterotoxin (LT) from Escherichia coli and Cholera toxin (CT) from Vibro cholerae are known to be potent mucosal immunogens. These toxins have 80% sequence homology and a similar tertiary structure (1 ,4 ), and both elicit
Methods for the Study of Intermolecular and Intramolecular Interactions Regulating CFTR Function
Caucasians in the United States (1 ). Excessive CFTR activity is implicated in cases of toxin-induced secretory diarrhea (e.g., by cholera toxin and heat stable Escherichia coli enterotoxin) that stimulate cAMP or cGMP production in the gut
Determining the Activity of Mucosal Adjuvants
immunization. Among promising mucosal adjuvants, mutants of cholera toxin and the closely related heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli present powerful tools, augmenting the local and systemic serum antibody response
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