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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
12个月
- 英文名:
Recombinant Mouse Beta-defensin 33 (Defb33)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10ug/20ug/50ug/100ug/200ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥69.0 |
|---|---|---|---|
| 规格: | 20ug | 产品价格: | ¥139.0 |
| 规格: | 50ug | 产品价格: | ¥209.0 |
| 规格: | 100ug | 产品价格: | ¥349.0 |
| 规格: | 200ug | 产品价格: | ¥419.0 |
| 规格: | 500ug | 产品价格: | ¥559.0 |
| 规格: | 1mg | 产品价格: | ¥699.0 |
Research Areas
OthersUniprot ID
Q30KN3Gene Names
Defb33Alternative Name(s)
Defensin, beta 33Lead Time
3-7 business daysOrganism
Mus musculus (Mouse)Source
E.coliExpression Region
21-62aaProtein Length
Full Length of Mature ProteinTag Info
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-taggedTarget Protein Sequence
RKRNSKFRPCEKMGGICKSQKTHGCSILPAECKSRYKHCCRLMW
24.9 kDaPurity
Greater than 85% as determined by SDS-PAGE.Form
Liquid or Lyophilized powderBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。
diabetic mouse model. Furthermore, the detection of peripheral autoreactive CD8+ T cells in this mouse model is indicative of beta cell killing and correlates with the development of diabetes. We describe two protocols that are helpful for the detection
FA Extraction of [insoluble] Amyloid beta from Mouse Brain
each sample individually for one minute on ice. Immerse the tip of the probe in the sample, then move tube up and down while sonicating. 3. Spin 400 μl at 135,000 x g for 1 hr at 4°C (50,000 rpm in a TLA 100.3 rotor). 4. Dilute 210 μl
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