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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Arabidopsis thaliana Beta-amylase 3, chloroplastic (BAM3)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Arabidopsis thaliana Beta-amylase 3, chloroplastic (BAM3)品名简称:
Recombinant Mouse-ear cress BAM3 protein货号:
CSB-EP515812DOA规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
BAM3Uniprot No.:
O23553别名:
1,4-alpha-D-glucan maltohydrolase (Beta-amylase 8) (Chloroplast beta-amylase) (CT-BMY) (BMY8) (CTBMY)种属:
Arabidopsis thaliana (Mouse-ear cress)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
59.4 kDa表达区域:
50-548aa氨基酸序列:
EMKFTHEKTFTPEGETLEKWEKLHVLSYPHSKNDASVPVFVMLPLDTVTMSGHLNKPRAMNASLMALKGAGVEGVMVDAWWGLVEKDGPMNYNWEGYAELIQMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDKSGRRNPEYISLGCDSVPVLRGRTPIQVYSDFMRSFRERFEGYIGGVIAEIQVGMGPCGELRYPSYPESNGTWRFPGIGEFQCYDKYMKSSLQAYAESIGKTNWGTSGPHDAGEYKNLPEDTEFFRRDGTWNSEYGKFFMEWYSGKLLEHGDQLLSSAKGIFQGSGAKLSGKVAGIHWHYNTRSHAAELTAGYYNTRNHDGYLPIAKMFNKHGVVLNFTCMEMKDGEQPEHANCSPEGLVKQVQNATRQAGTELAGENALERYDSSAFGQVVATNRSDSGNGLTAFTYLRMNKRLFEGQNWQQLVEFVKNMKEGGHGRRLSKEDTTGSDLYVGFVKGKIAENVEEAALV蛋白标签:
N-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Beta-amylase activity. No alpha-amylase activity. Involved in cold resistance. Mediates the accumulation of maltose upon freezing stress, thus contributing to the protection of the photosynthetic electron transport chain. Plays a role in the circadian-regulated starch degradation and maltose metabolism in chloroplasts, especially at night. More active on phosphorylated glucan. Interacts directly with starch or other alpha-1,4-glucan.功能2:
/文献:
"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome." Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S., Town C.D. Plant J. 89:789-804(2017)研究领域:
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文献和实验小,而且免疫原性弱,因此由它产生的抗体几乎可以忽略,如果用它作为重组蛋白标签就不需要考虑去掉由标签产生的抗体。另外,重组蛋白因为不存在修饰,也不存在高级结构,因此最终生产出来的抗体可能无法识别天然蛋白,难以用于某些实验。如果你怕麻烦不想生产蛋白,你可以去看看市场上有没有制备好的蛋白,这样可以节省不少时间和精力。 (3)人工合成多肽。很多蛋白属于家族蛋白,而这些家族内的蛋白相似度都很高,以Actin(肌动蛋白)家族来说,alpha-actin、beta-actin、gamma-actin之间的相似
和 Rubi3 [ 67, 68 ],以及从甘蔗中分离出的 ubi4 和 ubi9 [ 69 ] 启动子,所有这些启动子的表达活性与玉米 Ubil 或 CaMV35S 相当甚至更高。玉米 Ubil 驱动 GtAS 基因在转基因拟南芥、番茄、烟草和松树的根中也获得了很好的表达[70]。 Actin 基因的启动子是可以替代泛素的另一个理想启动子。水稻 Actin 启动子 ( Actl- D) 由 Act1 基因翻译起始密码子上游 2.1 kb 的一段序列组成。该序列包含了 GUS 高水平表达
cDNA Library Construction from Single Cells
numbers of cells. J. Cell Biochem. 39:1‐11. Skynner, M.J., Sim, J.A., and Herbison, A.E. 1999. Detection of estrogen receptor alpha and beta messenger ribonucleic acids in adult
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