alkB/alkB蛋白Recombinant Cauloba

纯度：

Greater than 85% as determined by SDS-PAGE.

基因名：

alkB

Uniprot No.：

B8GWW6

别名：

DNA oxidative demethylase AlkB

种属：

Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus)

蛋白长度：

Full Length

来源：

E.coli

分子量：

31.2 kDa

表达区域：

1-220aa

氨基酸序列：

MAVVRRAVAARGLQMIAKPLTVVPGFDVWPGLLDISAQRALVEAVLAGAEQAPFSNYRTAYGKPMSVAMTALGSLGWTSDARGYRYVDRHPETGRPWPDMPPALLDLWTVLGDPETPPDSCLVNLYRDGARMGLHQDRDEADPRFPVLSISLGDTAVFRIGGVNRKDPTRSLRLASGDVCRLLGPARLAFHGVDRILPG_S_SSLVPGGGRINLTLRRARTA

蛋白标签：

N-terminal 10xHis-tagged and C-terminal Myc-tagged

产品提供形式：

Liquid or Lyophilized powder

缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

货期：

3-7 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能：

Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable activity towards 1-methylguanine or 3-methylthymine. Accepts double-stranded and single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. Provides extensive resistance to alkylating agents such as MMS and DMS, but not to MMNG and MNU.By similarity

内毒素：

Not test.

SDS-PAGE:

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP489400DQP could indicate that this peptide derived from E.coli-expressed Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus) alkB.

LC-MS Image Description/Western Blot:

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Product types:

In Stock Protein

Biological_Activity:

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Research Areas:

Epigenetics and Nuclear Signaling

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Reference:

"An alkB gene homolog is differentially transcribed during the Caulobacter crescentus cell cycle." Colombi D., Gomes S.L. J. Bacteriol. 179:3139-3145(1997)

Function:

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