/alkB重组蛋白DNA oxidative demethy

英文名称：

Recombinant Caulobacter vibrioides Alpha-ketoglutarate-dependent dioxygenase AlkB homolog (alkB)

品名简称：

Recombinant Caulobacter vibrioides alkB protein

货号：

CSB-EP489400DQP

规格:

20μg

价格：

2328

LC-MS/Western Blot：

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SDS-PAGE：

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP489400DQP could indicate that this peptide derived from E.coli-expressed Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus) alkB.

纯度：

Greater than 85% as determined by SDS-PAGE.

内毒素：

Not test

生物活性：

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基因名：

alkB

Uniprot No.：

B8GWW6

别名：

DNA oxidative demethylase AlkB

种属：

Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus)

蛋白长度：

Full Length

来源：

E.coli

分子量：

31.2 kDa

表达区域：

1-220aa

氨基酸序列：

MAVVRRAVAARGLQMIAKPLTVVPGFDVWPGLLDISAQRALVEAVLAGAEQAPFSNYRTAYGKPMSVAMTALGSLGWTSDARGYRYVDRHPETGRPWPDMPPALLDLWTVLGDPETPPDSCLVNLYRDGARMGLHQDRDEADPRFPVLSISLGDTAVFRIGGVNRKDPTRSLRLASGDVCRLLGPARLAFHGVDRILPGSSSLVPGGGRINLTLRRARTA

蛋白标签：

N-terminal 10xHis-tagged and C-terminal Myc-tagged

产品提供形式：

Liquid or Lyophilized powder

缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

储存条件：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

货期：

3-7 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能1：

Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable activity towards 1-methylguanine or 3-methylthymine. Accepts double-stranded and single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. Provides extensive resistance to alkylating agents such as MMS and DMS, but not to MMNG and MNU.By similarity

功能2：

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文献：

"An alkB gene homolog is differentially transcribed during the Caulobacter crescentus cell cycle." Colombi D., Gomes S.L. J. Bacteriol. 179:3139-3145(1997)

研究领域：

Epigenetics and Nuclear Signaling

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