相关产品推荐更多 >

Recombinant-Pan-troglodytes-Cytochrome-c-oxidase-subunit-3MT-CO3Cytochrome c oxidase subunit 3 Alternative name(s): Cytochrome c oxidase polypeptide III
¥11102
G/G蛋白Recombinant R_a_bies v_i_rus Glycoprotein (G)重组蛋白GGlycoprotein蛋白
¥2328
/dhaA重组蛋白/Agrobacterium tumefaciens Haloalkane dehalogenase(dhaA)蛋白
¥2328
Recombinant-Mouse-Transmembrane-protein-151BTmem151bTransmembrane protein 151B
¥13762
Recombinant-Bacillus-PS3-ATP-synthase-subunit-catpEATP synthase subunit c Alternative name(s): ATP synthase F(0) sector subunit c F-type ATPase subunit c; F-ATPase subunit c Lipid-binding protein
¥9422
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Cytolethal distending toxin subunit C (cdtC)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Escherichia coli Cytolethal distending toxin subunit C (cdtC)品名简称:
Recombinant E.coli cdtC protein货号:
CSB-EP674965ENL规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
cdtCUniprot No.:
Q46670别名:
CDT C种属:
Escherichia coli蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
25.7 kDa表达区域:
16-181aa氨基酸序列:
CSSSQDSANNQIDELGKENNSLFTFRNIQSGLMIHNGLHQHGRETIGWEIVPVKTPEEALVTDQSGWIMIRTPNTDQCLGTPDGRNLLKMTCNSTAKKTLFSLIPSTTGAVQIKSVLSGLCFLDSKNSGLSFETGKCIADFKKPFEVVPQSHLWMLNPLNTESPII蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Part of the tripartite complex that is required for the CDT activity. CdtC, along with CdtA, probably forms a heterodimeric subunit required for the delivery of CdtB.功能2:
/文献:
"Cloning, sequencing, and expression of the Escherichia coli cytolethal distending toxin genes." Pickett C.L., Cottle D.L., Pesci E.C., Bikah G. Infect. Immun. 62:1046-1051(1994)研究领域:
Others请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Isolation of Membrane Rafts and Signaling Complexes
were visualized using Alexa Fluor 647 cholera toxin capped with anti-cholera toxin antibody. Co-localization of the C subunit of cytolethal distending toxin to rafts was determined using patching techniques.
摘要 基因重组蛋白在大肠杆菌中表达时,由于表达量高,往往形成无生物活性的包涵体。包涵体必须经过变性和复性的过程才能获得有活性的重组蛋白。如何提高基因重组蛋白质的复性率,是生物工程技术的一个研究热点。对近年来的重组蛋白质的复性方法做一评述,为研究蛋白质折叠以及复性技术的进一步应用提供依据。 关键词 重组蛋白 包涵体 复性 二硫键 到目前为止,人们表达的重组蛋白质已有4000多种,其中用E.coli表达的蛋白质要占90%以上,尽管基因重组技术为大规模生产目标蛋白质提供
表达容易形成不溶的聚集体,称为“包涵体”。大肠杆菌表达产生包涵体有以下优点[1,2]: 1) 以包涵体形式表达的蛋白质的浓度比较高,有时甚至可以达到细胞总蛋白含量的30% [3]; 2) 如果重组蛋白质以包涵体形式存在,由于包埋了酶攻击的位点,可以最大限度地抵抗蛋白质酶的攻击; 3) 包涵体表达的蛋白质没有活性,细胞破碎和以后的纯化步骤,不用考虑蛋白质的失活问题; 4) 包涵体形式表达的蛋白质与宿主细胞的其他蛋白质的分离比可溶蛋白质的分离方法简便,造价低,使用简单的离心或者过滤
技术资料暂无技术资料 索取技术资料






