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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Pyrroline-5-carboxylate reductase (proC)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Pyrroline-5-carboxylate reductase (proC)品名简称:
Recombinant Mycobacterium tuberculosis proC protein货号:
CSB-EP607537MVZ规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
proCUniprot No.:
P9WHU7别名:
P5C reductase;P5CR;PCA reductase种属:
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)蛋白长度:
Full Length来源:
E.coli分子量:
37.6 kDa表达区域:
1-295aa氨基酸序列:
MLFGMARIAIIGGGSIGEALLSGLLRAGRQVKDLVVAERMPDRANYLAQTYSVLVTSAADAVENATFVVVAVKPADVEPVIADLANATAAAENDSAEQVFVTVVAGITIAYFESKLPAGTPVVRAMPNAAALVGAGVTALAKGRFVTPQQLEEVSALFDAVGGVLTVPESQLDAVTAVSGSGPAYFFLLVEALVDAGVGVGLSRQVATDLAAQTMAGSAAMLLERMEQDQGGANGELMGLRVDLTASRLRAAVTSPGGTTAAALRELERGGFRMAVDAAVQAAKSRSEQLRITPE蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline.功能2:
/文献:
"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. Barrell B.G. Nature 393:537-544(1998)研究领域:
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文献和实验1-pyrroline-5-carboxylate reductase)以及编码脂肪、碳水化合物、核酸和维生素代谢中相关代谢酶的基因。 然而,有些真核细胞的酶需要翻译后加工,是不能应用E.coli异源互补法的。为了解决不能对植物基因进行蛋白翻译后加工修饰的问题,人们发展应用了酵母突变体的异源互补法。尽管用于互补的表达文库一般产生融合多肽,有些酶不能忍受任何N-端的延伸,但该法的筛选效率比抗体或杂交高2-3倍,尤其是对那些非常不容易被纯化或不稳定的蛋白(如膜蛋白等)来说,更是如此。应用酵母异源互补法用于分离
that, barring effects of the PCA fragment fusion, they would normally undergo. Assays can be performed in any cell type or model organism that can be transformed or transfected with gene expression DNA constructs. Here we focus on recent applications of PCA
日本VESSEL(威威)GT-P6EXD,GT-P6EXD(A
日本VESSEL(威威)气动螺丝刀: GT-S45.XD,GT-P4.5XD,GT-PLXD,GT-P6EXD,GT-P6EXD(A),GT-S6LXD,GT-P6LXD,GT-P60XD,GT-H2,GT-H4R,GT-H4PR,GT-PH4,GT-H4RC,GT-H5R,GT-H5P,GT-PH5,GT-H5RC,GT-S4.5DR,GT-P4.5DR,GT-PLP,GT-PLR,GT-PLRC,GT-PLZ,GT-P5LS,GT-PLHII,GT-P
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