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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG218.1 homolog
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG218.1 homolog品名简称:
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG218.1 homolog货号:
CSB-EP305096MLW规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
/Uniprot No.:
P75470别名:
/种属:
Mycoplasma pneumoniae (strain ATCC 29342 / M129)蛋白长度:
Full Length来源:
E.coli分子量:
48.0 kDa表达区域:
1-357aa氨基酸序列:
MTNDYQQLNYLVETDDEADIIIANLVKQLNELKEILLSLDNQDLEINQVNHRTTVHNTSSNTSNNSTSNNIADGNYNSNFFHNFSKETLQTQAKRGFLLLERCSLVGLQQLELEYLNVLGRSFESHQDKINFLVNLRDLTNDHLLDTEKIVSTLEQIFNVIGGTEYTPILNSFFNQSLNDPDPIQREIGLKQFVANLRQRFKTLLQKTNNSIQQLEAEIQIPTTHIKSDEVMFGPPDMNERLVLNDSETDAILRSIEAELESALQNKKQVVVTAVPPIAANLATDSISQETLEHNLNNTETVNTTTVTVTSASETQVRKPQISLRPIFQGNFPKRLSREDIQRYAHQLQELEQSNEG蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
"Proteome organization in a genome-reduced bacterium." Kuhner S., van Noort V., Betts M.J., Leo-Macias A., Batisse C., Rode M., Yamada T., Maier T., Bader S., Beltran-Alvarez P., Castano-Diez D., Chen W.H., Devos D., Guell M., Norambuena T., Racke I., Rybin V., Schmidt A. Gavin A.C. Science 326:1235-1240(2009)研究领域:
Others请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验糖,建议进行 Pre-clear;如果使用磁珠,此步可忽略 抗 X 抗体的轻链(25 kD)和重链(50 kD) 更侧重于如何在实验上进行优化(见下文) *注意:总蛋白上样量过多也会造成 非特异性结合,建议上样量为 500 ug-1 mg。上样之前一定要先用 BCA 定个量!定个量!定个量! pre-clear:上样前先用裂解液过一遍柱子,减少基质本身对蛋白的吸附 Output:在某些 co-IP 实验中,实验人员会把IP后的上清分别进行诱饵蛋白 X 和靶蛋白 Y 的 WB 检测
Subculture of Suspension Cell Lines
are obtained within 4 weeks. Mycoplasma colonies observed on agar plates have a `fried egg? appearance (see Mycoplasma pneumoniae',' ');">Figure 14). Materials 70% ethanol in water (Prod. No. R8382) Mycoplasma Pig Agar plates (in 5cm petri dishes
Size Price (RMB) AL0103050 (6 x 50ųl) 6,042.68 AL0103200 (6 x 200ųl) 19, 752.40 AL0103500 (6 x 500ųl) 45,092.65 CFPE700-新一代无细胞蛋白表达试剂盒(小麦胚芽) 新一代无细胞蛋白质表达试剂盒(小麦胚芽)是一种低成本、高灵活性、高产量重组蛋白表达系统,采用便捷的protocol可在一天
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