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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Colicin-E5 (col), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Escherichia coli Colicin-E5 (col), partial品名简称:
Recombinant E.coli col protein, partial货号:
CSB-CF323819ENL1规格:
20μg价格:
5268LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
colUniprot No.:
P18000别名:
/种属:
Escherichia coli蛋白长度:
Partial来源:
in vitro E.coli expression system分子量:
24.8 kDa表达区域:
74-180aa氨基酸序列:
LAKNKGKIPGLKIDQKIRGQMPERGWTEDDIKNTVSNGATGTSFDKRSPKKTPPDYLGRNDPATVYGSPGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
/研究领域:
Signal Transduction请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验限制性核酸内切酶和修饰酶产生的质粒 携带有编码合成限制性核酸内切酶和修饰酶的基因。 大肠杆菌素质粒 ( Col plasmid ,或称 Col 因子) 携带有产生大肠杆菌素( colicin )酶系基因的质粒,赋予大肠杆菌产生大肠杆菌素的能力。大肠杆菌素( colicin )是一种由 E.coli 的某些菌株所分泌的细菌素,具有通过抑制复制、转录、转译或能量
相关专题 大肠杆菌的基因工程 基因工程让一切皆有可能 一、原理 1、 E . coli 表达系统 E . coli 是重要的原核表达体系。在重组 基因转化入E . coli 菌株以后,通过温度的控制,诱导其在宿主菌内表达目的蛋白质,将表达样品进行SDS-PAGE 以检测表达蛋白质。 2、 外源基因的诱导表达 提高外源基因表达 水平的基本手段之一,就是将宿主菌的生长与外源基因的表达分成
culture 二倍体 细胞培养 Bacterium 细菌 Disinfection 消毒 Blastomyces dermatitidis 皮炎芽生菌 Enteroniavasive Escherichia col,EIEC 肠侵袭性大肠杆菌 Bordetella pertussis 百日咳杆菌 Endogenous infection 内源性感染 Borrelia 疏螺旋体属 El-Tor biotype 埃尔托生物型 B.recurrentis 回归热螺旋体 Endotoxin 内毒
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